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Characterization of a Novel Species of Legionella Isolated from a Healthcare Facility: Legionella resiliens sp. nov.
Cristino, Sandra; Pascale, Maria Rosaria; Marino, Federica; Derelitto, Carlo; Salaris, Silvano; Orsini, Massimiliano; Squarzoni, Stefano; Grottola, Antonella; Girolamini, Luna.
Afiliación
  • Cristino S; Department of Biological, Geological, and Environmental Sciences, University of Bologna, Via San Giacomo 12, 40126 Bologna, Italy.
  • Pascale MR; European Society of Clinical Microbiology and Infectious Diseases (ESCMID), Study Group for Legionella Infections (ESGLI), 4001 Basel, Switzerland.
  • Marino F; Department of Biological, Geological, and Environmental Sciences, University of Bologna, Via San Giacomo 12, 40126 Bologna, Italy.
  • Derelitto C; Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Viale del Risorgimento 2, 40136 Bologna, Italy.
  • Salaris S; Department of Biological, Geological, and Environmental Sciences, University of Bologna, Via San Giacomo 12, 40126 Bologna, Italy.
  • Orsini M; Department of Biological, Geological, and Environmental Sciences, University of Bologna, Via San Giacomo 12, 40126 Bologna, Italy.
  • Squarzoni S; Unit of Biostatistics, Epidemiology and Public Health, Department of Cardiac, Thoracic, Vascular Sciences, and Public Health, University of Padova, Via Loredan, 18, 35121 Padova, Italy.
  • Grottola A; Laboratory of Microbial Ecology and Genomics of Microorganisms, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy.
  • Girolamini L; Unit of Bologna, CNR Institute of Molecular Genetics "Luigi Luca Cavalli-Sforza", 40136 Bologna, Italy.
Pathogens ; 13(3)2024 Mar 14.
Article en En | MEDLINE | ID: mdl-38535593
ABSTRACT
Two Legionella-like isolates, 8cVS16T and 9fVS26, were isolated from a water distribution system (WDS) in a healthcare facility. Cells were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, motile, and exhibited a blue-white fluorescence under Wood's lamp at 365 nm. The strains grew in a range of 32-37 °C on BCYE with L-cysteine (Cys+), GVPC, and MWY agar medium, with a positive reaction for oxidase, catalase, and gelatinase. The dominant fatty acids were summed features 3 (C161ω7c/C161ω6c) (27.7%), C160 iso (17.5%), and C160 (16.3%), and Q13 as the major ubiquinone. The mip and rpoB gene sequences showed a similarity of 96.7% and 92.4%, with L. anisa (ATCC 35292T). The whole genomes sequencing (WGS) performed displayed a GC content of 38.21 mol% for both. The digital DNA-DNA hybridization (dDDH) analysis demonstrated the separation of the two strains from the phylogenetically most related L. anisa (ATCC 35292T), with ≤43% DNA-DNA relatedness. The Average Nucleotide Identity (ANI) between the two strains and L. anisa (ATCC 35292T) was 90.74%, confirming that the two isolates represent a novel species of the genus Legionella. The name proposed for this species is Legionella resiliens sp. nov., with 8cVS16T (=DSM 114356T = CCUG 76627T) as the type strain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pathogens Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza