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Ethanol leaf extracts of Anogeissus leiocarpus in antioxidants and hepatotoxic effects of Escherichia coli O157:H7 infected Swiss Mice.
Akharaiyi, Fred C; Ehis-Eriakha, Chioma B; Olagbemide, Peter T; Akemu, Stephen E.
Afiliación
  • Akharaiyi FC; Department of Microbiology, Edo State University Uzairue, KM 7 Auchi-Abuja Road, Edo State, Nigeria.
  • Ehis-Eriakha CB; Department of Microbiology, Edo State University Uzairue, KM 7 Auchi-Abuja Road, Edo State, Nigeria.
  • Olagbemide PT; Department of Biological Science, Afe Babalola University, P. M. B. 5454, Ado Ekiti, Ekiti State, Nigeria.
  • Akemu SE; Department of Microbiology, Edo State University Uzairue, KM 7 Auchi-Abuja Road, Edo State, Nigeria.
Biomedicine (Taipei) ; 14(1): 39-46, 2024.
Article en En | MEDLINE | ID: mdl-38533297
ABSTRACT

Introduction:

Diseases caused by bacteria can be managed with medicinal plants with rightful dosage that will not affect body physiology and organs.

Aim:

This research aimed to evaluate the antioxidants and the effects of Anogeisus leiocarpus on liver function. Materials and

methods:

Ethanol leaf extracts were processed for antioxidants and hepatotoxic effects using animal models. Group one (negative control) was given access to water and regular feed, group two (positive control) was dosed with 107 CFU/ml of Escherichia coli O157H7, and groups 3-6 were dosed with 107 E. coli O157H7 for 3 days and treated with extract concentrations of 12, 25, 50 and 100 mg/kg bw respectively for seven days.

Results:

Higher ascorbic acid values in Ferric reducing antioxidant property (FRAP) and Hydroxyl radical scavenging (HRS) were recorded in the positive control (0.05 ± 0.01, 41 ± 0.05) than in the extract-treated (0.02 ± 0.14, 30 ± 0.02). Increase in DPPH (47 ± 0.1268 ± 0.05 %), Free radical scavenging property (FRAP) (0.03 ± 0.02-0.08 ± 0.14 %), and HRS (38 ± 0.14-68 ± 0.12 %) was observed in the extract. The lipid peroxidation (LPO) of the quote was 78.51 ± 2.16, GSH was 36.18 ± 3.18, and catalase was 78.42 ± 4.713. In the extract-treated, decreased values were recorded for LPO ((108.36 ± 1.12-70.19 ± 1.68 µM/g), while increased values were observed in Glutathione (GSH) (25.11 ± 2.64-33.62 ± 1.35 µM/g), and catalase (54.18 ± 2.14-60.25 ± 1.4 µM/g). The values of negative control for Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and Alkaline phosphate (ALP) were lesser than what was received in the extract treated.

Conclusion:

The plant's traditional medicine usage is effective at low dosage and could be a suitable candidate for drug development which will not affect the body's physiology and organs. The subjecting of A. leiocarpus ethanol leaf extract to antioxidants assays and its effect on liver function have further proved its value in folklore medicine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicine (Taipei) Año: 2024 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicine (Taipei) Año: 2024 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: China