Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat.

Kopec, Przemyslaw; Krzewska, Monika; Plazek, Agnieszka

Kopec, Przemyslaw; Krzewska, Monika; Plazek, Agnieszka.

Afiliación

Kopec P; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. p.kopec@ifr-pan.edu.pl.
Krzewska M; The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland.
Plazek A; Department of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland.

Methods Mol Biol ; 2791: 113-119, 2024.

Article en En | MEDLINE | ID: mdl-38532098

ABSTRACT

ABSTRACT

Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining.

Asunto(s)

Fagopyrum; Proteoma; Proteoma/análisis; Proteómica; Focalización Isoeléctrica/métodos; Hojas de la Planta/química; Flores; Electroforesis en Gel Bidimensional/métodos; Geles; Concentración de Iones de Hidrógeno

Palabras clave

Colloidal Coomassie staining; Isoelectric focusing; Proteome analysis; SDS-PAGE; Two-dimensional gel electrophoresis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fagopyrum / Proteoma Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos

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