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Structure of the intact tail machine of Anabaena myophage A-1(L).
Yu, Rong-Cheng; Yang, Feng; Zhang, Hong-Yan; Hou, Pu; Du, Kang; Zhu, Jie; Cui, Ning; Xu, Xudong; Chen, Yuxing; Li, Qiong; Zhou, Cong-Zhao.
Afiliación
  • Yu RC; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Yang F; Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, China.
  • Zhang HY; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Hou P; Research Center for Intelligent Computing Platforms, Zhejiang Lab, Hangzhou, China.
  • Du K; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Zhu J; Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, China.
  • Cui N; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Xu X; Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, China.
  • Chen Y; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Li Q; Biomedical Sciences and Health Laboratory of Anhui Province, University of Science and Technology of China, Hefei, China.
  • Zhou CZ; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Nat Commun ; 15(1): 2654, 2024 Mar 26.
Article en En | MEDLINE | ID: mdl-38531972
ABSTRACT
The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the capsid. Structural analysis combined with biochemical assays further enable us to identify the dual hydrolytic activities of the baseplate hub, in addition to two host receptor binding domains in the tail fibers. Moreover, the structure of the intact A-1(L) also helps us to reannotate its genome. These findings will facilitate the application of A-1(L) as a chassis cyanophage in synthetic biology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anabaena / Myoviridae Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anabaena / Myoviridae Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido