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Single drop cytometry onboard the International Space Station.
Rea, Daniel J; Miller, Rachael S; Crucian, Brian E; Valentine, Russell W; Cristoforetti, Samantha; Bearg, Samuel B; Sipic, Zlatko; Cheng, Jamie; Yu, Rebecca; Calaway, Kimesha M; Eames, Dexter; Nelson, Emily S; Lewandowski, Beth E; Perusek, Gail P; Chan, Eugene Y.
Afiliación
  • Rea DJ; DNA Medicine Institute (DMI), Bedford, MA, USA.
  • Miller RS; rHEALTH, Bedford, MA, USA.
  • Crucian BE; KBR, Houston, TX, USA.
  • Valentine RW; Human Health and Performance Directorate, NASA Johnson Space Center, Houston, TX, USA.
  • Cristoforetti S; ZIN Technologies, Middleburg Heights, OH, USA.
  • Bearg SB; European Space Agency, Paris, France.
  • Sipic Z; DNA Medicine Institute (DMI), Bedford, MA, USA.
  • Cheng J; rHEALTH, Bedford, MA, USA.
  • Yu R; DNA Medicine Institute (DMI), Bedford, MA, USA.
  • Calaway KM; rHEALTH, Bedford, MA, USA.
  • Eames D; DNA Medicine Institute (DMI), Bedford, MA, USA.
  • Nelson ES; rHEALTH, Bedford, MA, USA.
  • Lewandowski BE; DNA Medicine Institute (DMI), Bedford, MA, USA.
  • Perusek GP; rHEALTH, Bedford, MA, USA.
  • Chan EY; ZIN Technologies, Middleburg Heights, OH, USA.
Nat Commun ; 15(1): 2634, 2024 Mar 25.
Article en En | MEDLINE | ID: mdl-38528030
ABSTRACT
Real-time lab analysis is needed to support clinical decision making and research on human missions to the Moon and Mars. Powerful laboratory instruments, such as flow cytometers, are generally too cumbersome for spaceflight. Here, we show that scant test samples can be measured in microgravity, by a trained astronaut, using a miniature cytometry-based analyzer, the rHEALTH ONE, modified specifically for spaceflight. The base device addresses critical spaceflight requirements including minimal resource utilization and alignment-free optics for surviving rocket launch. To fully enable reduced gravity operation onboard the space station, we incorporated bubble-free fluidics, electromagnetic shielding, and gravity-independent sample introduction. We show microvolume flow cytometry from 10 µL sample drops, with data from five simultaneous channels using 10 µs bin intervals during each sample run, yielding an average of 72 million raw data points in approximately 2 min. We demonstrate the device measures each test sample repeatably, including correct identification of a sample that degraded in transit to the International Space Station. This approach can be utilized to further our understanding of spaceflight biology and provide immediate, actionable diagnostic information for management of astronaut health without the need for Earth-dependent analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vuelo Espacial / Ingravidez Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vuelo Espacial / Ingravidez Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido