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Synergistic Effect of Electrical and Biochemical Stimulation on Human iPSC-Derived Neural Differentiation in a Microfluidic Electrode Array Chip.
Kim, Na Yeon; Choi, Yoon Young; Kim, Tae Hyeon; Ha, Jang Ho; Kim, Tae-Hyung; Kang, Taewook; Chung, Bong Geun.
Afiliación
  • Kim NY; Department of Biomedical Engineering, Sogang University, Seoul 04107, Korea.
  • Choi YY; Institute of Integrated Biotechnology, Sogang University, Seoul 04107, Korea.
  • Kim TH; Department of Mechanical Engineering, Sogang University, Seoul 04107, Korea.
  • Ha JH; Department of Mechanical Engineering, Sogang University, Seoul 04107, Korea.
  • Kim TH; School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.
  • Kang T; Institute of Integrated Biotechnology, Sogang University, Seoul 04107, Korea.
  • Chung BG; Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea.
ACS Appl Mater Interfaces ; 16(13): 15730-15740, 2024 Apr 03.
Article en En | MEDLINE | ID: mdl-38527279
ABSTRACT
Neural differentiation is crucial for advancing our understanding of the nervous system and developing treatments for neurological disorders. The advanced methods and the ability to manipulate the alignment, proliferation, and differentiation of stem cells are essential for studying neuronal development and synaptic interactions. However, the utilization of human induced pluripotent stem cells (iPSCs) for disease modeling of neurodegenerative conditions may be constrained by the prolonged duration and uncontrolled cell differentiation required for functional neural cell differentiation. Here, we developed a microfluidic chip to enhance the differentiation and maturation of specific neural lineages by placing aligned microelectrodes on the glass surface to regulate the neural differentiation of human iPSCs. The utilization of electrical stimulation (ES) in conjunction with neurotrophic factors (NF) significantly enhanced the efficiency in generating functional neurons from human iPSCs. We also observed that the simultaneous application of NF and ES to human iPSCs promoted their differentiation and maturation into functional neurons while increasing synaptic interactions. Our research demonstrated the effect of combining NF and ES on human iPSC-derived neural differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos