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Catharanthus roseus-assisted bio-fabricated zinc oxide nanoparticles for promising antibacterial potential against Klebsiella pneumoniae.
Sultana, Sumreen; Ashwini, Bagepalli Shivaram; Ansari, Mohammad Azam; Alomary, Mohammad N; Jamous, Yahya F; Ravikiran, Tekupalli; Niranjana, Siddapura Ramachandrappa; Begum, M Yasmin; Siddiqua, Ayesha; Lakshmeesha, Thimappa Ramachandrappa.
Afiliación
  • Sultana S; Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560056, India.
  • Ashwini BS; Department of Microbiology, Shri Atal Bihari Vajpayee Medical College & Research Institute, Bengaluru, 560001, India.
  • Ansari MA; Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia. maansari@iau.edu.sa.
  • Alomary MN; Advanced Diagnostic and Therapeutic Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh, 11442, Saudi Arabia.
  • Jamous YF; Vaccine and Bioprocessing Center, King Abdulaziz City for Science and Technology (KACST), Riyadh, 11442, Saudi Arabia.
  • Ravikiran T; Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560056, India.
  • Niranjana SR; Department of Studies in Biotechnology, University of Mysore, Mysore, 570006, India.
  • Begum MY; Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
  • Siddiqua A; Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
  • Lakshmeesha TR; Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, 560056, India. lakshmeesha@bub.ernet.in.
Bioprocess Biosyst Eng ; 47(8): 1259-1269, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38526617
ABSTRACT
This study emphasized on the synthesis of zinc oxide nanoparticles (ZnO NPs) in an environmentally friendly manner from the extract of Catharanthus roseus leaves and its antibacterial assessment against the pneumonia-causing pathogen Klebsiella pneumoniae. This simple and convenient phytosynthesis approach is found to be beneficial over conventional methods, wherein plants serve as excellent reducing, capping, and stabilizing agents that enables the formation of ZnO NPs without the use of harmful chemicals. The formation of ZnO NPs was confirmed through several characterization techniques such as UV-visible spectroscopy, XRD, FT-IR, SEM, HR-TEM, and EDX. XRD analysis revealed high polycrystallinity with crystallite size of approximately 13 nm. SEM and HR-TEM revealed the hexagonal structure of ZnO NPs with the particle size range of 20-50 nm. The EDX shows the elemental purity without any impurity. Furthermore, the antibacterial efficacy by the technique of disc diffusion exhibited clear inhibition zones in ZnO NPs-treated discs. In addition, 125 µg/mL of ZnO NP concentration showed minimum inhibition by the microbroth dilution method. The potent inhibitory activity was further validated with trypan blue dye exclusion and fluorescence microscopy. Finally, SEM examination confirmed the efficient antibacterial potential of ZnO NPs through disruption of the intact morphology of Klebsiella pneumoniae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Catharanthus / Nanopartículas del Metal / Klebsiella pneumoniae / Antibacterianos Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Catharanthus / Nanopartículas del Metal / Klebsiella pneumoniae / Antibacterianos Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania