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Cadaver preservative properties of a solution composed of honey, ethyl alcohol, liquid paraffin, distilled water and citric acid: Experiments on rabbit cadavers.
Demiraslan, Yasin; Gürbüz, Iftar; Özbek, Mehmet; Sahan Yapicier, Özlem; Karaca, Harun; Özgel, Özcan; Öner, Hakan.
Afiliación
  • Demiraslan Y; Department of Anatomy, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
  • Gürbüz I; Department of Anatomy, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
  • Özbek M; Department of Histology and Embryology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
  • Sahan Yapicier Ö; Republic of Turkey Ministry of Agriculture and Forestry Veterinary Control, Central Research Institute, Bacteriology Diagnostic Laboratory, Ankara, Turkey.
  • Karaca H; Department of Histology and Embryology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
  • Özgel Ö; Department of Anatomy, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
  • Öner H; Department of Histology and Embryology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Anat Histol Embryol ; 53(3): e13032, 2024 May.
Article en En | MEDLINE | ID: mdl-38525664
ABSTRACT
The objective of this study is to assess the efficacy of a solution including honey, ethyl alcohol, liquid paraffin, distilled water and citric acid (HEFS) as a preservative for rabbit cadavers, serving as a potential substitute for formaldehyde. The cadavers underwent preservation using three distinct solutions 10% formalin, 35% alcohol and HEFS. The cadavers were subjected to a total of four sampling events, occurring at 4-month intervals, in order to collect specimens for microanatomical, histological, microbiological, mycological, colourimetric, texture and odour analysis. In terms of hardness, suitability for dissection and joint mobility metrics, the cadavers fixed with HEFS had superior qualities to those fixed with formalin. The fixation quality of HEFS for histological analyses was deemed acceptable, except kidney and intestinal tissues. In texture analysis, differences only in the elasticity parameter (p < 0.05) in the same sampling period. A total of 10 (13.9) bacteria isolates were identified among which, Metasolibacillus meyeri 3 (30%) was predominantly followed by Staphylococcus aureus 2 (20%), Bacillus siamensis, Bacillus subtilis, Pseudarthrobacter oxydans, Bacillus licheniformis, Bacillus subtilis subsp. subtilis with a proportion of 1 (10%), respectively, by both microbiological and molecular analysis. However, no anaerobic bacteria and fungi were isolated. A considerable percentage of the students had the perception that HEFS was appropriate for utilization in laboratory settings due to its absence of unpleasant odours and detrimental impact on ocular and respiratory functions. In conclusion, we consider that HEFS may serve as a viable substitute for formalin solution in the preservation of rabbit cadavers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Aceite Mineral / Miel Límite: Animals / Humans Idioma: En Revista: Anat Histol Embryol Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus / Aceite Mineral / Miel Límite: Animals / Humans Idioma: En Revista: Anat Histol Embryol Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Alemania