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Development of a rapid-fire drug screening method by probe electrospray ionization tandem mass spectrometry for human urine (RaDPi-U).
Hisatsune, Kazuaki; Murata, Tasuku; Taniguchi, Masaru; Asano, Tomomi; Ogata, Koretsugu; Iguchi, Akira; Zaitsu, Kei.
Afiliación
  • Hisatsune K; Forensic Science Laboratory, Aichi Prefectural Police Headquarters, 2-1-1, Sannomaru, Naka-ku, Nagoya, 460-8502, Japan. hisat26@gmail.com.
  • Murata T; Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto, 604-8511, Japan.
  • Taniguchi M; Nagoya City Public Health Research Institute, Sakurazaka, Moriyama-ku, Nagoya, 463-8585, Japan.
  • Asano T; Department of Human Life and Environment, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
  • Ogata K; Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto, 604-8511, Japan.
  • Iguchi A; Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 7, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8567, Japan.
  • Zaitsu K; Research Laboratory On Environmentally-conscious Developments and Technologies [E-code], National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, 305-8567, Japan.
Anal Bioanal Chem ; 416(10): 2503-2513, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38523158
ABSTRACT
Drug screening tests are mandatory in the search for drugs in forensic biological samples, and immunological methods and mass spectrometry (e.g., gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry) are commonly used for that purpose. However, these methods have some drawbacks, and developing new screening methods is required. In this study, we develop a rapid-fire drug screening method by probe electrospray ionization tandem mass spectrometry (PESI-MS/MS), which is an ambient ionization mass spectrometry method, for human urine, named RaDPi-U. RaDPi-U is carried out in three

steps:

(1) mixing urine with internal standard (IS) solution and ethanol, followed by vortexing; (2) pipetting the mixture onto a sample plate for PESI; and (3) rapid-fire analysis by PESI-MS/MS. RaDPi-U targets 40 forensically important drugs, which include illegal drugs, hypnotics, and psychoactive substances. The analytical results were obtained within 3 min because of the above-mentioned simple workflow of RaDPi-U. The calibration curves of each analyte were constructed using the IS method, and they were quantitatively valid, resulting in good linearity (0.972-0.999) with a satisfactory lower limit of detection and lower limit of quantitation (0.01-7.1 ng/mL and 0.02-21 ng/mL, respectively). Further, both trueness and precisions were 28% or less, demonstrating the high reliability and repeatability of the method. Finally, we applied RaDPi-U to three postmortem urine specimens and successfully detected different drugs in each urine sample. The practicality of the method is proven, and RaDPi-U will be a strong tool as a rapid-fire drug screening method not only in forensic toxicology but also in clinical toxicology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masa por Ionización de Electrospray / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: Anal Bioanal Chem Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masa por Ionización de Electrospray / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: Anal Bioanal Chem Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania