Your browser doesn't support javascript.
loading
Phage resistance in Klebsiella pneumoniae and bidirectional effects impacting antibiotic susceptibility.
Nang, Sue C; Lu, Jing; Yu, Heidi H; Wickremasinghe, Hasini; Azad, Mohammad A K; Han, Meiling; Zhao, Jinxin; Rao, Gauri; Bergen, Phillip J; Velkov, Tony; Sherry, Norelle; McCarthy, David T; Aslam, Saima; Schooley, Robert T; Howden, Benjamin P; Barr, Jeremy J; Zhu, Yan; Li, Jian.
Afiliación
  • Nang SC; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Lu J; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Yu HH; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Wickremasinghe H; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Azad MAK; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Han M; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Zhao J; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Rao G; Division of Pharmacotherapy and Experimental Therapeutics, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, USA.
  • Bergen PJ; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • Velkov T; Biomedicine Discovery Institute, Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
  • Sherry N; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
  • McCarthy DT; Department of Civil Engineering, Monash University, Clayton, Victoria, Australia.
  • Aslam S; Division of Infectious Diseases and Global Public Health, Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Schooley RT; Division of Infectious Diseases and Global Public Health, Department of Medicine, University of California San Diego, La Jolla, CA, USA.
  • Howden BP; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
  • Barr JJ; School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
  • Zhu Y; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
  • Li J; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia. Electronic address: jian.li@monash.edu.
Clin Microbiol Infect ; 30(6): 787-794, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38522841
ABSTRACT

OBJECTIVES:

Bacteriophage (phage) therapy is a promising anti-infective option to combat antimicrobial resistance. However, the clinical utilization of phage therapy has been severely compromised by the potential emergence of phage resistance. Although certain phage resistance mechanisms can restore bacterial susceptibility to certain antibiotics, a lack of knowledge of phage resistance mechanisms hinders optimal use of phages and their combination with antibiotics.

METHODS:

Genome-wide transposon screening was performed with a mutant library of Klebsiella pneumoniae MKP103 to identify phage pKMKP103_1-resistant mutants. Phage-resistant phenotypes were evaluated by time-kill kinetics and efficiency of plating assays. Phage resistance mechanisms were investigated with adsorption, one-step growth, and mutation frequency assays. Antibiotic susceptibility was determined with broth microdilution and population analysis profiles.

RESULTS:

We observed a repertoire of phage resistance mechanisms in K pneumoniae, such as disruption of phage binding (fhuATn and tonBTn), extension of the phage latent period (mnmETn and rpoNTn), and increased mutation frequency (mutSTn and mutLTn). Notably, in contrast to the prevailing view that phage resistance re-sensitizes antibiotic-resistant bacteria, we observed a bidirectional steering effect on bacterial antibiotic susceptibility. Specifically, rpoNTn increased susceptibility to colistin while mutSTn and mutLTn increased resistance to rifampicin and colistin.

DISCUSSION:

Our findings demonstrate that K pneumoniae employs multiple strategies to overcome phage infection, which may result in enhanced or reduced antibiotic susceptibility. Mechanism-guided phage steering should be incorporated into phage therapy to better inform clinical decisions on phage-antibiotic combinations.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Pruebas de Sensibilidad Microbiana / Klebsiella pneumoniae / Antibacterianos Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Pruebas de Sensibilidad Microbiana / Klebsiella pneumoniae / Antibacterianos Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido