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Rapid identification,fluconazole and micafungin susceptibility testing of Candida species from blood culture by a short incubation method.
de Souza, Ândrea Celestino; Pereira, Dariane Castro; Barth, Patricia Orlandi; Roesch, Eliane Würdig; Lutz, Larissa; Aquino, Valério Rodrigues; Goldani, Luciano Zubaran.
Afiliación
  • de Souza ÂC; Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil. Electronic address: acsouza93@hotmail.com.
  • Pereira DC; Unidade de Microbiologia, Serviço de Diagnóstico Laboratorial - Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
  • Barth PO; Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
  • Roesch EW; Unidade de Microbiologia, Serviço de Diagnóstico Laboratorial - Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
  • Lutz L; Unidade de Microbiologia, Serviço de Diagnóstico Laboratorial - Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
  • Aquino VR; Unidade de Microbiologia, Serviço de Diagnóstico Laboratorial - Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
  • Goldani LZ; Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
Diagn Microbiol Infect Dis ; 109(2): 116271, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38522370
ABSTRACT
This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92.05 %. Regarding the rapid broth microdilution for Fluconazole and Micafungin, the agreement was 97.06 % (p<0.001) and 100 % (p<0.001), and the Kappa coefficient was 0.91 (p<0.001) and 1.0 (p<0.001), respectively. To conclude, both rapid methods showed to be reproducible, inexpensive, easy to perform and time-saving. Thus, these methodologies could be useful to guide and adjust empirical antifungal therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Pruebas de Sensibilidad Microbiana / Fluconazol / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Equinocandinas / Lipopéptidos / Cultivo de Sangre / Micafungina / Antifúngicos Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Pruebas de Sensibilidad Microbiana / Fluconazol / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Equinocandinas / Lipopéptidos / Cultivo de Sangre / Micafungina / Antifúngicos Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos