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S100A6 mediated epithelial-mesenchymal transition affects chemosensitivity of colorectal cancer to oxaliplatin.
Zhang, Chunying; Zeng, Menglu; Xu, Yihan; Huang, Bihan; Shi, Pengchong; Zhu, Xianjin; Cao, Yingping.
Afiliación
  • Zhang C; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.
  • Zeng M; Department of Clinical Laboratory, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynaecology and Paediatrics, Fujian Medical University, Fuzhou, China.
  • Xu Y; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.
  • Huang B; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.
  • Shi P; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.
  • Zhu X; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China. Electronic address: zxj5027667@163.com.
  • Cao Y; Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China. Electronic address: caoyingping@aliyun.com.
Gene ; 914: 148406, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38521111
ABSTRACT

PURPOSE:

To investigate the mechanism by which S100 calcium-binding protein A6 (S100A6) affects colorectal cancer (CRC) cells to oxaliplatin (L-OHP) chemotherapy, and to explore new strategies for CRC treatment.

METHODS:

S100A6 expression was assessed in both parental and L-OHP-resistant CRC cells using western blotting, quantitative real-time polymerase chain reaction (qRT-PCR), and enzyme-linked immunosorbent assays (ELISA). Lentiviral vectors were utilized to induce the knockdown of S100A6 expression, followed by comprehensive evaluations of cell proliferation, apoptosis, and epithelial-mesenchymal transition (EMT). Additionally, RNA-seq analysis was conducted to identify genes associated with the knockdown of S100A6.

RESULTS:

Elevated S100A6 expression in CRC tissues correlated with an adverse prognosis in patients with CRC. Higher expression of S100A6 was also observed in L-OHP-resistant CRC cells, which showed enhanced proliferation, migration, invasion, and antiapoptotic capabilities. Notably, the knockdown of S100A6 expression resulted in decreased proliferation, increased apoptosis, and suppression of EMT and tumorigenicity in L-OHP-resistant CRC cells. Transcriptome sequencing reveals a noteworthy association between S100A6 and vimentin expression. Application of the EMT agonist, transforming growth factor ß (TGF-ß), induces EMT in CRC cells. S100A6 expression positively correlates with TGF-ß expression. TGF-ß facilitated the expression of EMT-related molecules and reduced the chemosensitivity of L-OHP in S100A6-knockdown cells.

CONCLUSION:

In conclusion, the knockdown of S100A6 may overcome the L-OHP resistance of CRC cells by modulating EMT.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Apoptosis / Proteínas de Ciclo Celular / Resistencia a Antineoplásicos / Proliferación Celular / Transición Epitelial-Mesenquimal / Proteína A6 de Unión a Calcio de la Familia S100 / Oxaliplatino Límite: Animals / Female / Humans / Male Idioma: En Revista: Gene Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Apoptosis / Proteínas de Ciclo Celular / Resistencia a Antineoplásicos / Proliferación Celular / Transición Epitelial-Mesenquimal / Proteína A6 de Unión a Calcio de la Familia S100 / Oxaliplatino Límite: Animals / Female / Humans / Male Idioma: En Revista: Gene Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos