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Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China.
Jiang, Qing-Qing; Zhu, Juan-Juan; Fan, Shu-Ling; Hou, Ya-Ping; Hu, Xie-Ying; Shi, Jie; Wu, Lei; Luo, Ying.
Afiliación
  • Jiang QQ; Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200050, China.
  • Zhu JJ; Chaozhou Hybribio Limited Corporation, Guangdong, China.
  • Fan SL; Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
  • Hou YP; Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200050, China.
  • Hu XY; Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200050, China.
  • Shi J; Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210037, China.
  • Wu L; Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200050, China.
  • Luo Y; Department of Clinical Laboratory, Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200050, China.
Asian Biomed (Res Rev News) ; 18(1): 11-17, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38515630
ABSTRACT

Background:

Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.

Objective:

Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.

Methods:

A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss-related genes including GJB2, GJB3, SLC26A4, and the mitochondrial gene MT-RNR1. This method involved PCR amplification systems and a hybridization platform.

Results:

The technique can detect 13 hotspot mutations of 4 hearing loss-related genes. And a total of 213 blood samples were used to evaluate the availability of this method.

Discussion:

Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss-related genes in a Chinese population.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Asian Biomed (Res Rev News) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Tailandia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Asian Biomed (Res Rev News) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Tailandia