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Integration of a hamper pad on test strips for improved sensitivity of carbendazim detection.
Makkala, Pumnatthiga; Ruantip, Sirowan; Buakeaw, Anumart; Chaiyo, Sudkate; Khongchareonporn, Nanthika.
Afiliación
  • Makkala P; Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand.
  • Ruantip S; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand.
  • Buakeaw A; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand.
  • Chaiyo S; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence for Food and Water Risk Analysis (FAWRA), Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electr
  • Khongchareonporn N; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence for Food and Water Risk Analysis (FAWRA), Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electr
Talanta ; 273: 125911, 2024 Jun 01.
Article en En | MEDLINE | ID: mdl-38508129
ABSTRACT
Lateral flow immunoassays (LFIAs) are widely used to determine carbendazim (CBZ) residues in food products due to their advantages of low cost, ease and rapid use, on-site detection capability. However, conventional LFIAs have low detection sensitivity. Although improvements have been made to increase the sensitivity, it is not sufficient. Here, a hamper pad, polyvinyl alcohol coated on a nitrocellulose membrane, was integrated to enhance the sensitivity of LFIA for CBZ detection. The hamper pad was inserted between the conjugated and nitrocellulose pads to delay the flow rate, thereby increasing the possibility of the antibody and target analyte binding. This platform exhibited a fourfold sensitivity increase in CBZ detection compared with the conventional LFIA, and its limit of detection was 1.6 ng/mL. In addition, a single-step operation was successfully applied to detect CBZ in rice (white rice, brown rice, sticky rice, and paddy) and soybean samples, with acceptable recoveries of 93.6%-120.0%. This novel device was compared to the standard high-performance liquid chromatography method, which shows high accuracy with a Kappa coefficient of 0.91. Therefore, improved sensitivity with a rapid, simple, and inexpensive device could facilitate the detection of CBZ residues in agricultural products for on-field screening and improved user-friendliness.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bencimidazoles / Nanopartículas del Metal Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bencimidazoles / Nanopartículas del Metal Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos