Your browser doesn't support javascript.
loading
Antimicrobial and antibiofilm activity of highly soluble polypyrrole against methicillin-resistant Staphylococcus aureus.
Rosa, Danillo Sales; Oliveira, Samily Aquino de Sá; Souza, Renata de Faria Silva; de França, Chirles Araujo; Pires, Isabelle Caroline; Tavares, Márcio Rennan Santos; de Oliveira, Helinando Pequeno; da Silva Júnior, Fernando Antônio Gomes; Moreira, Maria Aparecida Scatamburlo; de Barros, Mariana; de Menezes, Gustavo Batista; Antunes, Maísa Mota; Azevedo, Vasco Ariston de Carvalho; Naue, Carine Rosa; da Costa, Mateus Matiuzzi.
Afiliación
  • Rosa DS; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • Oliveira SAS; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • Souza RFS; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • de França CA; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • Pires IC; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • Tavares MRS; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • de Oliveira HP; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • da Silva Júnior FAG; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
  • Moreira MAS; Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
  • de Barros M; Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil.
  • de Menezes GB; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
  • Antunes MM; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
  • Azevedo VAC; Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
  • Naue CR; Hospital Universitário da Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56304-205, Brazil.
  • da Costa MM; Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco 56300-000, Brazil.
J Appl Microbiol ; 135(4)2024 Apr 01.
Article en En | MEDLINE | ID: mdl-38503568
ABSTRACT

AIMS:

The purpose was to evaluate the antimicrobial activity of highly soluble polypyrrole (Hs-PPy), alone or combined with oxacillin, as well as its antibiofilm potential against methicillin-resistant Staphylococcus aureus strains. Furthermore, the in silico inhibitory mechanism in efflux pumps was also investigated. METHODS AND

RESULTS:

Ten clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and two reference strains were used. Antimicrobial activity was determined by broth microdilution, and the combination effect with oxacillin was evaluated by the checkerboard assay. The biofilm formation capacity of MRSA and the interference of Hs-PPy were evaluated. The inhibitory action of Hs-PPy on the efflux pump was evaluated in silico through molecular docking. Hs-PPy showed activity against the isolates, with inhibitory action between 62.5 and 125 µg ml-1 and bactericidal action at 62.5 µg ml-1, as well as synergism in association with oxacillin. The isolates ranged from moderate to strong biofilm producers, and Hs-PPy interfered with the formation of this structure, but not with mature biofilm. There was no in silico interaction with the efflux protein EmrD, the closest homolog to NorA.

CONCLUSIONS:

Hs-PPy interferes with biofilm formation by MRSA, has synergistic potential, and is an efflux pump inhibitor.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus Resistente a Meticilina / Antiinfecciosos Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus Resistente a Meticilina / Antiinfecciosos Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido