The transcription factor MYB1 activates DGAT2 transcription to promote triacylglycerol accumulation in sacha inchi (Plukenetia volubilis L.) leaves under heat stress.

Yang, Tianquan; Niu, Qian; Dai, Huan; Tian, Xiaoling; Ma, Junchao; Pritchard, Hugh W; Lin, Liang; Yang, Xiangyun

Yang, Tianquan; Niu, Qian; Dai, Huan; Tian, Xiaoling; Ma, Junchao; Pritchard, Hugh W; Lin, Liang; Yang, Xiangyun.

Afiliación

Yang T; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Niu Q; College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
Dai H; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Tian X; Institute of International Rivers and Eco-Security, Yunnan University, Kunming, 650500, China.
Ma J; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Pritchard HW; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Lin L; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China. Electronic address: linliang@mail.kib.ac.cn.
Yang X; Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China. Electronic address: yxy@mail.kib.ac.cn.

Plant Physiol Biochem ; 208: 108517, 2024 Mar.

Article en En | MEDLINE | ID: mdl-38503190

ABSTRACT

ABSTRACT

Triacylglycerol (TAG) accumulation is frequently triggered in vegetative tissues experiencing heat stress, which may increases plant basal plant thermo-tolerance by sequestering the toxic lipid intermediates that contribute to membrane damage or cell death under stress conditions. However, stress-responsive TAG biosynthesis and the underlying regulatory mechanisms are not fully understood. Here, we investigated the lipidomic and transcriptomic landscape under heat stress in the leaves of sacha inchi (Plukenetia volubilis L.), an important oilseed crop in tropical regions. Under heat stress (45 °C), the content of polyunsaturated TAGs (e.g., TAG182 and TAG183) and total TAGs were significantly higher, while those of unsaturated sterol esters, including ZyE 284, SiE 182 and SiE 183, were dramatically lower. Transcriptome analysis showed that the expression of PvDGAT2-2, encoding a type II diacylglycerol acyltransferase (DGAT) that is critical for TAG biosynthesis, was substantially induced under heat stress. We confirmed the function of PvDGAT2-2 in TAG production by complementing a yeast mutant defective in TAG biosynthesis. Importantly, we also identified the heat-induced transcription factor PvMYB1 as an upstream activator of PvDGAT2-2 transcription. Our findings on the molecular mechanism leading to TAG biosynthesis in leaves exposed to heat stress have implications for improving the biotechnological production of TAGs in vegetative tissues, offering an alternative to seeds.

Asunto(s)

Aceites de Plantas; Factores de Transcripción; Triglicéridos/metabolismo; Factores de Transcripción/genética; Factores de Transcripción/metabolismo; Aceites de Plantas/metabolismo; Hojas de la Planta/genética; Hojas de la Planta/metabolismo; Respuesta al Choque Térmico/genética

Palabras clave

Diacylglycerol acyltransferase genes; Heat stress; Lipidomics; Sacha inchi; Transcriptome; Triacylglycerol

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Aceites de Plantas Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Francia

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