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Overexpression of miR-22-3p and miR-29c-3p in CFU-Hill colonies is related to senescence process among children with low birth weight.
Souza, Paula R P; Thomazini, Fernanda; Souza, Livia V; Freitas, Carla L; do Carmo Franco, Maria.
Afiliación
  • Souza PRP; Physiology Department, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • Thomazini F; Post-Graduate Program in Translational Medicine, Medicine Department, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • Souza LV; Physiology Department, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • Freitas CL; Post-Graduate Program in Translational Medicine, Medicine Department, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • do Carmo Franco M; Nephrology Division, Medicine Department, School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
Pediatr Res ; 96(2): 480-485, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38499628
ABSTRACT

BACKGROUND:

The current study mainly focused on provide further insights into the association of the miR-22-3p and miR-29c-3p expression in CFU-Hill colonies with birth weight and senescence process in children.

METHODS:

This cross-sectional study evaluated 61 children (32 boys, 29 girls). The CFU-Hill colonies number was evaluated in vitro by cell culture technique and senescence was detected by ß-galactosidase (SA-ß-Gal) assay. Expression of miR-22-3p and miR-29c-3p isolated from CFU-Hill colonies were detected using quantitative real-time polymerase chain reaction.

RESULTS:

Birth weight was correlated with both CFU-Hill colonies and %SA-ß-Gal positive staining. Multivariate linear regression analysis revealed that the senescence was a predictor of the lower CFU-Hill colonies number, while only the birth weight was a predictor of senescence of CFU-Hill colonies. Overexpression of miR-22-3p and miR-29c-3p was observed in CFU-Hill colonies isolated from children with low birth weight (LBW). Interestingly, we found a significant correlation between %SA-ß-Gal cells staining positive for both miR-22-3p and miR-29c-3p.

CONCLUSION:

The LBW is associated with decreased CFU-Hill colonies number and high senescence of these cells. The overexpression of miR-22-3p and miR-29c-3p may be partially responsible for this alteration due to regulation of several pathways related to the senescence process. IMPACT The study establishes a significant correlation between birth weight and the number of CFU-Hill colonies, suggesting that birth weight could be a predictive biomarker for vascular health in children. Data indicates that cellular senescence is a predictor of reduced CFU-Hill colony numbers. This suggests that the aging process of these cells could be an important factor in understanding the vascular health issues in children with low birth weight. The overexpression of miR-22-3p and miR-29c-3p in children with low birth weight and their correlation with increased cellular senescence highlight these microRNAs as possible molecular mechanisms influencing the aging of CFU-Hill colonies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recién Nacido de Bajo Peso / Senescencia Celular / MicroARNs Límite: Child, preschool / Female / Humans / Infant / Male / Newborn Idioma: En Revista: Pediatr Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recién Nacido de Bajo Peso / Senescencia Celular / MicroARNs Límite: Child, preschool / Female / Humans / Infant / Male / Newborn Idioma: En Revista: Pediatr Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos