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An improved chromosome-level genome assembly of perennial ryegrass (Lolium perenne L.).
Chen, Yutang; Kölliker, Roland; Mascher, Martin; Copetti, Dario; Himmelbach, Axel; Stein, Nils; Studer, Bruno.
Afiliación
  • Chen Y; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Universitaetstrasse 2, 8092, Zurich, Switzerland.
  • Kölliker R; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Universitaetstrasse 2, 8092, Zurich, Switzerland.
  • Mascher M; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Seeland, Germany.
  • Copetti D; Arizona Genomics Institute, School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
  • Himmelbach A; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Seeland, Germany.
  • Stein N; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Seeland, Germany.
  • Studer B; Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Universitaetstrasse 2, 8092, Zurich, Switzerland.
GigaByte ; 2024: gigabyte112, 2024.
Article en En | MEDLINE | ID: mdl-38496214
ABSTRACT
This work is an update and extension of the previously published article "Ultralong Oxford Nanopore Reads Enable the Development of a Reference-Grade Perennial Ryegrass Genome Assembly" by Frei et al. The published genome assembly of the doubled haploid perennial ryegrass (Lolium perenne L.) genotype Kyuss (Kyuss v1.0) marked a milestone for forage grass research and breeding. However, order and orientation errors may exist in the pseudo-chromosomes of Kyuss, since barley (Hordeum vulgare L.), which diverged 30 million years ago from perennial ryegrass, was used as the reference to scaffold Kyuss. To correct for structural errors possibly present in the published Kyuss assembly, we de novo assembled the genome again and generated 50-fold coverage high-throughput chromosome conformation capture (Hi-C) data to assist pseudo-chromosome construction. The resulting new chromosome-level assembly Kyuss v2.0 showed improved quality with high contiguity (contig N50 = 120 Mb), high completeness (total BUSCO score = 99%), high base-level accuracy (QV = 50), and correct pseudo-chromosome structure (validated by Hi-C contact map). This new assembly will serve as a better reference genome for Lolium spp. and greatly benefit the forage and turf grass research community.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: GigaByte Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: GigaByte Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: China