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Assessment of vitamin D status and vitamin D receptor polymorphism in Egyptian children with Type 1 diabetes.
Mostafa, Eman A; Abo Hashish, Maha M A; Ismail, Nagwa Abdallah; Hasanin, Hasanin M; Hasanin, Rasha M; Wahby, Aliaa Ahmed; Ashmawy, Ingy; Abd El Aziz, Shereen Hamdy; Wahed, Mai Magdy Abdel.
Afiliación
  • Mostafa EA; Department of Pediatrics, National Research Center, Affiliation ID: 60014618, Cairo, Egypt. Electronic address: ea.moustafa@nrc.sci.eg.
  • Abo Hashish MMA; Department of Pediatrics, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Ismail NA; Department of Pediatrics, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Hasanin HM; Department of Pediatrics, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Hasanin RM; Department of Pediatrics, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Wahby AA; Department of Clinical and Chemical Pathology, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Ashmawy I; Department of Clinical and Chemical Pathology, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Abd El Aziz SH; Department of Clinical and Chemical Pathology, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
  • Wahed MMA; Department of Clinical and Chemical Pathology, National Research Center, Affiliation ID: 60014618, Cairo, Egypt.
J Genet Eng Biotechnol ; 22(1): 100343, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38494252
ABSTRACT

BACKGROUND:

The endocrine system of vitamin D regulates about 3 % of the human genome. Vitamin D exerts its actions via a nuclear vitamin D receptor (VDR) which in turn regulates insulin secretion from the pancreas. VDR gene polymorphisms could have an impact on how autoimmune illnesses like Type 1 diabetes mellitus (T1DM) develop. We aimed to explore the relation between T1DM and VDR gene polymorphisms in Egyptian diabetic children and their siblings.

METHODS:

Enzyme-linked immunosorbent assay was used to quantify 25(OH) vitamin D in the study, which had 179 participants (group 1 = 85 diabetic children, group 2 = 57 siblings of the patients, group 3 = 37 healthy controls). Real-time polymerase chain reaction (RT-PCR) was used to analyze the genotyping of the VDR gene polymorphisms Apa-I (rs7975232), Fok-I (rs2228570), Taq-I (rs731236) and Bsm-I (rs1544410).

RESULTS:

The mean serum 25(OH) vitamin D levels was significantly lower in T1DM patients (14.99 ± 9.24 ng/mL) and siblings (16.31 ± 7.96 ng/mL) compared to the controls (19.48 ± 7.42 ng/mL) (p = 0.031). The genotypes distribution of VDR Fok-I (rs2228570) and Bsm-I (rs1544410) polymorphisms showed a significant difference between patients, siblings and controls as P = 0.001 and 0.026 respectively, while the VDR ApaI and TaqI polymorphisms did not. FokI-A allele frequency was significantly lower in T1DM patients and siblings than in controls (p < 0.001). FokI-AA genotype had a statistical significant higher vitamin D levels than other genotypes with p value of 0.024.

CONCLUSION:

Our study found that T1DM children had lower vitamin D levels, and VDR FokI and BsmI gene polymorphisms were linked to T1DM in Egyptian children. Determining the relationship between vitamin D levels and VDR polymorphisms, particularly the FokI and other genetic analyses may aid in the early diagnosis of T1DM in children.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Genet Eng Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Genet Eng Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos