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CircRNA HLCS regulates lens epithelial cell apoptosis via miR-338-3p/BPNT1 axis.
Sun, Lianyi; Li, Fengzhi; Bai, Shuwei; Bi, Chunchao.
Afiliación
  • Sun L; Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, No. 21 Jiefang Road, Xi'an, 710004, China.
  • Li F; Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, No. 21 Jiefang Road, Xi'an, 710004, China.
  • Bai S; Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, No. 21 Jiefang Road, Xi'an, 710004, China. shuweibai1982@126.com.
  • Bi C; Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, No. 21 Jiefang Road, Xi'an, 710004, China.
Int Ophthalmol ; 44(1): 142, 2024 Mar 17.
Article en En | MEDLINE | ID: mdl-38493427
ABSTRACT

PURPOSE:

The purpose of this study was to investigate the effect of circ_HLCS on age-related cataract (ARC).

METHODS:

Circ_HLCS, microRNA (miR)-338-3p, and bisphosphate 3'-nucleotidase 1 (BPNT1) were quantified by quantitative real-time polymerase chain reaction or western blot. Cell proliferation and cell viability were assessed by the 5-Ethynyl-2'-deoxyuridinr and cell counting kit-8 assays. Cell apoptosis was detected by flow cytometry. Targeted correlations among circ_HLCS, miR-338-3p, and BPNT1 were verified by the dual-luciferase reporter and RNA pull-down assays.

RESULTS:

circ_HLCS was diminished in ARC tissues and UV-treated SRA01/04 cells. Elevated content of circ_HLCS undermined UV-induced cell proliferation inhibition and apoptosis. Mechanistically, circ_HLCS directly targeted miR-338-3p, and circ_HLCS regulated BPNT1 expression through miR-338-3p. Furthermore, reduction of miR-338-3p ameliorated UV-induced SRA01/04 cell damage by increasing BPNT1 expression.

CONCLUSION:

Taken together, these data suggested that circ_HLCS inhibited apoptosis of UV-treated SRA01/04 cells by miR-338-3p/BPNT1 axis. Therefore, circ_HLCS might be a potential therapeutic target for ARC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Cristalino Límite: Humans Idioma: En Revista: Int Ophthalmol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Cristalino Límite: Humans Idioma: En Revista: Int Ophthalmol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos