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CRISPR activation screens: navigating technologies and applications.
Clark, Teleri; Waller, Matthew A; Loo, Lipin; Moreno, Cesar L; Denes, Christopher E; Neely, G Gregory.
Afiliación
  • Clark T; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
  • Waller MA; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
  • Loo L; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
  • Moreno CL; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
  • Denes CE; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
  • Neely GG; Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia. Electronic address: greg.neely@sydney.edu.au.
Trends Biotechnol ; 42(8): 1017-1034, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38493051
ABSTRACT
Clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) has become an integral part of the molecular biology toolkit. CRISPRa genetic screens are an exciting high-throughput means of identifying genes the upregulation of which is sufficient to elicit a given phenotype. Activation machinery is continually under development to achieve greater, more robust, and more consistent activation. In this review, we offer a succinct technological overview of available CRISPRa architectures and a comprehensive summary of pooled CRISPRa screens. Furthermore, we discuss contemporary applications of CRISPRa across broad fields of research, with the aim of presenting a view of exciting emerging applications for CRISPRa screening.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas / Sistemas CRISPR-Cas / Edición Génica Límite: Animals / Humans Idioma: En Revista: Trends Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas / Sistemas CRISPR-Cas / Edición Génica Límite: Animals / Humans Idioma: En Revista: Trends Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido