Effects of aerobic exercise on the regulation of mitochondrial carrier homolog-2 and its influence on the catabolic and anabolic activity of lipids in the mesenteric adipose tissue of obese mice.

de Melo, Diego Gomes; da Cruz Rodrigues, Vivian Cristina; de Sá Pereira, Gustavo José; de Campos, Thais Dantis Pereira; Dos Santos Canciglieri, Raphael; Pauli, José Rodrigo; da Silva, Adelino Sanchez Ramos; da Costa Fernandes, Célio Junior; de Moura, Leandro Pereira

de Melo, Diego Gomes; da Cruz Rodrigues, Vivian Cristina; de Sá Pereira, Gustavo José; de Campos, Thais Dantis Pereira; Dos Santos Canciglieri, Raphael; Pauli, José Rodrigo; da Silva, Adelino Sanchez Ramos; da Costa Fernandes, Célio Junior; de Moura, Leandro Pereira.

Afiliación

de Melo DG; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil.
da Cruz Rodrigues VC; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil.
de Sá Pereira GJ; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil.
de Campos TDP; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil.
Dos Santos Canciglieri R; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil.
Pauli JR; Laboratory of Molecular Biology of Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil; Laboratory of Cell Signaling, Obesity and Comorbidities Research Center (OCRC), University of Campinas, Campinas, São Paulo, Brazil.
da Silva ASR; Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
da Costa Fernandes CJ; Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University - UNESP, Botucatu, São Paulo, Brazil.
de Moura LP; Exercise Cellular Biology Laboratory, University of Campinas, Limeira, Brazil; Laboratory of Cell Signaling, Obesity and Comorbidities Research Center (OCRC), University of Campinas, Campinas, São Paulo, Brazil. Electronic address: mouralp@unicamp.br.

Life Sci ; 345: 122567, 2024 May 15.

Article en En | MEDLINE | ID: mdl-38492919

ABSTRACT

ABSTRACT

The aim was to understand the direct impact of aerobic short-term exercise on lipid metabolism, specifically in regulating the mitochondrial carrier homolog 2 (MTCH2) and how it interferes with lipid metabolism in mesenteric adipose tissue. Swiss mice were divided into three groups control, sedentary obese, and exercised obese. The obese groups were induced into obesity for fourteen weeks of a high-fat diet, and the trained submitted to seven aerobic exercise sessions. The exercise proved the significant increase of the pPerilipin-1, a hormone-sensitive lipase gene, and modulates lipid metabolism by increasing the expression of Mtch2 and acetyl Co-A carboxylase, perhaps occurring as feedback to regulate lipid metabolism in adipose tissue. In conclusion, we demonstrate, for the first time, how aerobic physical exercise increases Mtch2 transcription in mesenteric adipose tissue. This increase was due to changes in energy demand caused by exercise, confirmed by observing the significant reduction in mesenteric adipose tissue mass in the exercised group. Also, we showed that physical exercise increased the phosphorylative capacity of PLIN1, a protein responsible for the degradation of fatty acids in the lipid droplet, providing acyl and glycerol for cellular metabolism. Although our findings demonstrate evidence of MTCH2 as a protein that regulates lipid homeostasis, scant knowledge exists concerning the signaling of the MTCH2 pathway in regulatingfatty acid metabolism. Therefore, unveiling the means of molecular signaling of MTCH2 demonstrates excellent potential for treating obesity.

Asunto(s)

Tejido Adiposo; Metabolismo de los Lípidos; Proteínas de Transporte de Membrana Mitocondrial; Obesidad; Condicionamiento Físico Animal; Animales; Ratones; Tejido Adiposo/metabolismo; Dieta Alta en Grasa/efectos adversos; Lípidos; Ratones Obesos; Proteínas de Transporte de Membrana Mitocondrial/metabolismo; Obesidad/metabolismo; Condicionamiento Físico Animal/fisiología; Metabolismo de los Lípidos/genética; Metabolismo de los Lípidos/fisiología

Palabras clave

Aerobic training; Inflammation; Mesenteric adipose tissue; Mitochondria; Obesity

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Tejido Adiposo / Proteínas de Transporte de Membrana Mitocondrial / Metabolismo de los Lípidos / Obesidad Límite: Animals Idioma: En Revista: Life Sci Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos

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