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Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity.
Lehr, François-Xavier; Pavletic, Bruno; Glatter, Timo; Heimerl, Thomas; Moeller, Ralf; Niederholtmeyer, Henrike.
Afiliación
  • Lehr FX; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
  • Pavletic B; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.
  • Glatter T; German Aerospace Center, Institute of Aerospace Medicine, Aerospace Microbiology, Cologne, Germany.
  • Heimerl T; Technical University of Braunschweig, Faculty of Life Sciences, Braunschweig, Germany.
  • Moeller R; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
  • Niederholtmeyer H; Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.
NPJ Microgravity ; 10(1): 30, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38491014
ABSTRACT
On-demand biomanufacturing has the potential to improve healthcare and self-sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi-protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled "astropharmacy".
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Microgravity Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Microgravity Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos