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Total coliforms, microbial diversity and multiple characteristics of Salmonella in soil-irrigation water-fresh vegetable system in Shaanxi, China.
Yang, Qiuping; Chen, Jin; Dai, Jinghan; He, Yuanjie; Wei, Kexin; Gong, Mengqing; Chen, Qinquan; Sheng, Huanjing; Su, Li; Liu, Lisha; Chen, Jia; Bai, Li; Cui, Shenghui; Yang, Baowei.
Afiliación
  • Yang Q; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Chen J; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Dai J; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • He Y; College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Wei K; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Gong M; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Chen Q; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Sheng H; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Su L; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Liu L; China National Center for Food Safety Risk Assessment, Beijing 100022, China.
  • Chen J; College of Chemical Technology, Shijiazhuang University, Shijiazhuang 050035, China. Electronic address: chenjia_0311@163.com.
  • Bai L; China National Center for Food Safety Risk Assessment, Beijing 100022, China. Electronic address: baili@cfsa.net.cn.
  • Cui S; National Institutes for Food and Drug Control, Beijing 100050, China. Electronic address: cuishenghui@aliyun.com.
  • Yang B; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: ybw090925@163.com.
Sci Total Environ ; 924: 171657, 2024 May 10.
Article en En | MEDLINE | ID: mdl-38490413
ABSTRACT
Global occurrences of foodborne disease outbreaks have been documented, involving fresh agricultural produce contaminated by various pathogens. This contamination can occur at any point in the supply chain. However, studies on the prevalence of total coliforms, Salmonella and microbial diversity in vegetable and associated environments are limited. This study aimed to assess 1) the number of total coliforms (n = 299) and diversity of microbial communities (n = 52); 2) the prevalence, antibiotic susceptibility, genomic characteristics, and potential transmission relationships of Salmonella in soil-irrigation water-vegetable system (n = 506). Overall, 84.28 % samples were positive to total coliforms, with most frequently detected in soil (100 %), followed by irrigation water (79.26 %) and vegetables (62.00 %). A seasonal trend in coliform prevalence was observed, with significantly higher levels in summer (P < 0.05). Detection rates of Salmonella in soil, vegetable and irrigation water were 2.21 %, 4.74 % and 9.40 %. Fourteen serotypes and sequence types (STs) were respectively annotated in 56 Salmonella isolates, ST13 S. Agona (30.36 %, 17/56), ST469 S. Rissen (25.00 %, 14/56), and ST36 S. Typhimurium (12.50 %, 7/56) were dominant serotypes and STs. Thirty-one (55.36 %) isolates were multi-drug resistant, and the resistance was most frequently found to ampicillin (55.36 %, 31/56), followed by to sulfamethoxazole (51.79 %, 29/56) and tetracycline (50.00 %, 28/56). The genomic characteristics and antibiotic resistance patterns of Salmonella isolates from soil, vegetables, and irrigation water within a coherent geographical locale exhibited remarkable similarities, indicating Salmonella may be transmitted among these environments or have a common source of contamination. Microbial alpha diversity indices in soil were significantly higher (P < 0.05) than that in vegetable and irrigation water. The microbial phylum in irrigation water covered that in the vegetable, demonstrating a significant overlap in the microbial communities between the vegetables and the irrigation water.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Suelo / Verduras Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Suelo / Verduras Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos