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Genome-Wide Association Study Reveals Polygenic Architecture for Limber Pine Quantitative Disease Resistance to White Pine Blister Rust.
Liu, Jun-Jun; Sniezko, Richard A; Houston, Sydney; Krakowski, Jodie; Alger, Genoa; Benowicz, Andy; Sissons, Robert; Zamany, Arezoo; Williams, Holly; Kegley, Angelia; Rancourt, Benjamin.
Afiliación
  • Liu JJ; Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
  • Sniezko RA; U.S. Department of Agriculture Forest Service, Dorena Genetic Resource Center, 34963 Shoreview Drive, Cottage Grove, OR 97424, U.S.A.
  • Houston S; Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
  • Krakowski J; Independent Consultant, Box 774, Coleman, AB, T0K 0M0, Canada.
  • Alger G; Parks Canada, Waterton Lakes National Park, Alberta, T0K 2M0, Canada.
  • Benowicz A; Alberta Forestry and Parks, Government of Alberta, Edmonton, T6H 5T6, Canada.
  • Sissons R; Parks Canada, Waterton Lakes National Park, Alberta, T0K 2M0, Canada.
  • Zamany A; Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
  • Williams H; Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
  • Kegley A; U.S. Department of Agriculture Forest Service, Dorena Genetic Resource Center, 34963 Shoreview Drive, Cottage Grove, OR 97424, U.S.A.
  • Rancourt B; Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
Phytopathology ; 114(7): 1626-1636, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38489164
ABSTRACT
Development of durable resistance effective against a broad range of pathotypes is crucial for restoration of pathogen-damaged ecosystems. This study dissected the complex genetic architecture for limber pine quantitative disease resistance (QDR) to Cronartium ribicola using a genome-wide association study. Eighteen-month-old seedlings were inoculated for resistance screening under controlled conditions. Disease development was quantitatively assessed for QDR-related traits over 4 years postinoculation. To reveal the genomic architecture contributing to QDR-related traits, a set of genes related to disease resistance with genome-wide distribution was selected for targeted sequencing for genotyping of single-nucleotide polymorphisms (SNPs). The genome-wide association study revealed a set of SNPs significantly associated with quantitative traits for limber pine QDR to white pine blister rust, including number of needle spots and stem cankers, as well as survival 4 years postinoculation. The peaks of marker-trait associations displayed a polygenic pattern, with genomic regions as potential resistant quantitative trait loci, distributed over 10 of the 12 linkage groups (LGs) of Pinus. None of them was linked to the Cr4-controlled major gene resistance previously mapped on LG08. Both normal canker and bole infection were mapped on LG05, and the associated SNPs explained their phenotypic variance up to 52%, tagging a major resistant quantitative trait locus. Candidate genes containing phenotypically associated SNPs encoded putative nucleotide-binding site leucine-rich repeat proteins, leucine-rich repeat-receptor-like kinase, cytochrome P450 superfamily protein, heat shock cognate protein 70, glutamate receptor, RNA-binding family protein, and unknown protein. The confirmation of resistant quantitative trait loci broadens the genetic pool of limber pine resistance germplasm for resistance breeding.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Basidiomycota / Polimorfismo de Nucleótido Simple / Pinus / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Basidiomycota / Polimorfismo de Nucleótido Simple / Pinus / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos