Your browser doesn't support javascript.
loading
Expression of Staphylococcus aureus translation elongation factor P is regulated by a stress-inducible promotor.
Zheng, Xingxing; Sun, Xiuhui; Xiang, Weiwei; Ni, Haiyan; Zou, Long; Long, Zhong-Er.
Afiliación
  • Zheng X; Nanchang Key Laboratory of Microbial Resources Exploitation and Utilization From Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
  • Sun X; Chongren County Center for Disease Prevention and Control, Chongren, 344203, China.
  • Xiang W; Nanchang Key Laboratory of Microbial Resources Exploitation and Utilization From Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
  • Ni H; Nanchang Key Laboratory of Microbial Resources Exploitation and Utilization From Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
  • Zou L; Nanchang Key Laboratory of Microbial Resources Exploitation and Utilization From Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
  • Long ZE; Nanchang Key Laboratory of Microbial Resources Exploitation and Utilization From Poyang Lake Wetland, College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China. longzhonger@163.com.
Antonie Van Leeuwenhoek ; 117(1): 54, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38489110
ABSTRACT
Translation elongation factor P, expressed by the efp gene, is a conserved protein closely related to bacterial virulence and environmental stress regulation responses, however, little is known about the efp gene expression regulations. Here, the strain of Staphylococcus aureus subsp. aureus NCTC 8325 was taken as the research object and cultured under different conditions, including different culture temperatures, pH, and antibiotics, to study the expression of the efp gene in S. aureus by qRT-PCR, the results showed that the expression of the efp gene is upregulated under high temperature (40 °C), acidic (pH 5.4) or alkaline (pH 9.4) culture conditions, but upregulated early and downregulated later under the conditions of 0.5 MIC antibiotics (chloramphenicol at the final concentration of 2 µg/mL and vancomycin at the final concentration of 0.25 µg/mL), indicating that the efp promoter in S. aureus is inducible. The efp promoter sequence and structure in S. aureus were predicted by bioinformatics methods, and the predicted promoter was validated by constructing a promoter-probe vector and a series of promoter mutants, the results showed that the efp promoter sequence in S. aureus, named Pro, located in 1,548,179-1,548,250 of the S. aureus genome (NC_007795.1), and the sequence of - 10 element is CCTTATAGT, - 35 element is TTTACT. The results above could lay a foundation for screening transcription factors involved in the expression of the efp gene and then exploring the transcriptional regulation mechanism of EF-P in S. aureus.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Factores de Elongación de Péptidos Límite: Humans Idioma: En Revista: Antonie Van Leeuwenhoek Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus / Factores de Elongación de Péptidos Límite: Humans Idioma: En Revista: Antonie Van Leeuwenhoek Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos