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An optrode array for spatiotemporally-precise large-scale optogenetic stimulation of deep cortical layers in non-human primates.
Clark, Andrew M; Ingold, Alexander; Reiche, Christopher F; Cundy, Donald; Balsor, Justin L; Federer, Frederick; McAlinden, Niall; Cheng, Yunzhou; Rolston, John D; Rieth, Loren; Dawson, Martin D; Mathieson, Keith; Blair, Steve; Angelucci, Alessandra.
Afiliación
  • Clark AM; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA.
  • Ingold A; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA.
  • Reiche CF; Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT, USA.
  • Cundy D; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA.
  • Balsor JL; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA.
  • Federer F; Department of Ophthalmology and Visual Science, Moran Eye Institute, University of Utah, Salt Lake City, UT, USA.
  • McAlinden N; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK.
  • Cheng Y; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK.
  • Rolston JD; Departments of Neurosurgery and Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.
  • Rieth L; Department of Neurosurgery, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Dawson MD; Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV, USA.
  • Mathieson K; Feinstein Institute for Medical Research, Manhasset, NY, USA.
  • Blair S; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK.
  • Angelucci A; SUPA, Institute of Photonics, Department of Physics, University of Strathclyde, Glasgow, UK.
Commun Biol ; 7(1): 329, 2024 Mar 14.
Article en En | MEDLINE | ID: mdl-38485764
ABSTRACT
Optogenetics has transformed studies of neural circuit function, but remains challenging to apply to non-human primates (NHPs). A major challenge is delivering intense, spatiotemporally-precise, patterned photostimulation across large volumes in deep tissue. Such stimulation is critical, for example, to modulate selectively deep-layer corticocortical feedback circuits. To address this need, we have developed the Utah Optrode Array (UOA), a 10×10 glass needle waveguide array fabricated atop a novel opaque optical interposer, and bonded to an electrically addressable µLED array. In vivo experiments with the UOA demonstrated large-scale, spatiotemporally precise, activation of deep circuits in NHP cortex. Specifically, the UOA permitted both focal (confined to single layers/columns), and widespread (multiple layers/columns) optogenetic activation of deep layer neurons, as assessed with multi-channel laminar electrode arrays, simply by varying the number of activated µLEDs and/or the irradiance. Thus, the UOA represents a powerful optoelectronic device for targeted manipulation of deep-layer circuits in NHP models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Optogenética / Neuronas Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Optogenética / Neuronas Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido