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Novel mutation in efflux pump Rv1258c (Tap) gene in drug resistant clinical isolates of Mycobacterium tuberculosis in Iran.
Farzaneh, ShimaSadat; Norouzi, Fatemeh; Fazeli, Hossein; Salehi, Mahshid; Safari, Marzieh; Nasr Esfahani, Bahram.
Afiliación
  • Farzaneh S; Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Norouzi F; Department of Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
  • Fazeli H; Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Salehi M; Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Safari M; Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Nasr Esfahani B; Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Infect Dev Ctries ; 18(2): 243-250, 2024 Feb 29.
Article en En | MEDLINE | ID: mdl-38484347
ABSTRACT

INTRODUCTION:

Tuberculosis (TB) remains a serious public health problem worldwide. Drug-resistant TB is considered a major and growing global threat. Despite the great variety of described mutations in Mycobacterium tuberculosis (MTB) resistance genes, the mechanisms of drug resistance are still controversial. Recently, a report on the role of efflux pump genes in drug resistance added to this complexity. Therefore, a thorough understanding of efflux pump genes in drug-resistant TB clinical isolates is needed.

METHODOLOGY:

We performed molecular analysis of the efflux pump gene (Rv1258c) in 33 drug-resistant and 20 drug-sensitive clinical MTB isolates by sequencing the amplicons' targets in both the forward and reverse directions.

RESULTS:

A novel mutation of the Rv1258c gene was identified at G442A (Ala148Thr) in rifampicin mono-resistant clinical strain, as compared to the H37Rv reference strain. In addition, a cytosine nucleotide insertion was found between the positions 580 and 581 (denominated Tap580) in two drug-sensitive strains at identical gene positions.

CONCLUSIONS:

These results indicated the possibility of mutation in the efflux pump genes and the important role of Tap efflux pump genes in drug-resistant MTB isolates. However, further research is required to determine the direct association of these mutations with resistant MTB.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Infect Dev Ctries Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Italia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Infect Dev Ctries Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Italia