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TMEM2 suppresses TLR3-mediated IFN-ß/ISG56/CXCL10 expression in BEAS-2B bronchial epithelial cells.
Kobori, Yuri; Tachizaki, Mayuki; Imaizumi, Tadaatsu; Tanaka, Yusuke; Seya, Kazuhiko; Miki, Yasuo; Kawaguchi, Shogo; Matsumiya, Tomoh; Tobisawa, Yuki; Ohyama, Chikara; Tasaka, Sadatomo.
Afiliación
  • Kobori Y; Department of Respiratory Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifucho, Hirosaki, 036-8562, Japan. yukobori@hirosaki-u.ac.jp.
  • Tachizaki M; Department of Vascular and Inflammatory Medicine, Biomedical Research Center, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Imaizumi T; Department of Vascular and Inflammatory Medicine, Biomedical Research Center, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Tanaka Y; Department of Respiratory Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifucho, Hirosaki, 036-8562, Japan.
  • Seya K; Department of Vascular and Inflammatory Medicine, Biomedical Research Center, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Miki Y; Department of Neuropathology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Kawaguchi S; Department of Vascular and Inflammatory Medicine, Biomedical Research Center, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Matsumiya T; Department of Vascular and Inflammatory Medicine, Biomedical Research Center, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Tobisawa Y; Department of Urology, Gifu University Hospital, Gifu, Japan.
  • Ohyama C; Department of Advanced Transplant and Regenerative Medicine, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Tasaka S; Department of Respiratory Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifucho, Hirosaki, 036-8562, Japan.
Mol Biol Rep ; 51(1): 417, 2024 Mar 14.
Article en En | MEDLINE | ID: mdl-38483660
ABSTRACT

BACKGROUND:

Bronchial epithelial cells are at the front line of viral infections. Toll-like receptor 3 (TLR3) cascade causes the expression of interferon (IFN)-ß and IFN-stimulated genes (ISGs), which in turn induce an antiviral response. Members of the transmembrane protein (TMEM) family are expressed in various cell types. Although the prognostic value of TMEM2 in various cancers has been reported, its association with infectious diseases remains unknown. In this study, we investigated the effects of TMEM2 on antiviral immunity in BEAS-2B bronchial epithelial cells. METHODS AND

RESULTS:

TMEM2 protein was found in the cytoplasm of normal human bronchial epithelial cells and differed between organs using immunohistochemistry. Cultured BEAS-2B cells were transfected with TMEM2 siRNA, followed by administration of TLR3 ligand polyinosinic-polycytidylic acid (poly IC) or recombinant human (r(h)) IFN-ß. The expression of TMEM2, IFN-ß, ISG56, C-X-C motif chemokine ligand 10 (CXCL10) and hyaluronan were evaluated appropriately by western blotting, quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. TMEM2 expression was not altered by poly IC stimulation. Knockdown of TMEM2 increased poly IC-induced expression of IFN-ß, CXCL10, and ISG56, while IFN-ß-induced expression of ISG56 and CXCL10 were not changed by TMEM2 knockdown. The hyaluronan concentration in the medium was decreased by either TMEM2 knockdown or poly IC, but additive or synergistic effects were not observed.

CONCLUSIONS:

TMEM2 knockdown enhanced TLR3-mediated IFN-ß, CXCL10, and ISG56 expression in BEAS-2B cells. This implies that TMEM2 suppresses antiviral immune responses and prevents tissue injury in bronchial epithelial cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor Toll-Like 3 / Ácido Hialurónico Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor Toll-Like 3 / Ácido Hialurónico Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos