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Metabolism of phenolic compounds catalyzed by Tomato CYP736A61.
Mai, Thanh Dat; Kim, Hyun Min; Park, Seo Young; Ma, Sang Hoon; Do, Ju Hui; Choi, Won; Jang, Hye Min; Hwang, Hyeon Bae; Song, Eun Gyeong; Shim, Jae Sung; Joung, Young Hee.
Afiliación
  • Mai TD; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Kim HM; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Park SY; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Ma SH; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Do JH; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Choi W; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Jang HM; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Hwang HB; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Song EG; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea.
  • Shim JS; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea; Institute of Synthetic Biology for Carbon Neutralization, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea. Electronic address: js
  • Joung YH; School of Biological Sciences and Technology, Chonnam National University, 77 Yongbong-ro, Buk-ku, Gwangju 61186, Republic of Korea. Electronic address: yhjoung@chonnam.ac.kr.
Enzyme Microb Technol ; 176: 110425, 2024 May.
Article en En | MEDLINE | ID: mdl-38479200
ABSTRACT
Cytochrome P450s (CYPs) regulate plant growth and stress responses by producing diverse primary and secondary metabolites. However, the function of many plant CYPs remains unknown because, despite their structural similarity, predicting the enzymatic activity of CYPs is difficult. In this study, one member of the CYP736A subfamily (CYP736A61) from tomatoes was isolated and characterized its enzymatic functions. CYP736A61 was successfully expressed in Escherichia coli through co-expression with molecular chaperones. The purified CYP736A61 showed hydroxylation activity toward 7-ethoxycoumarin, producing 7-hydroxycoumarin or 3-hydroxy 7-ethoxycoumarin. Further substrate screening revealed that dihydrochalcone and stilbene derivates (resveratrol and polydatin) are the substrates of CYP736A61. CYP736A61 also mediated the hydroxylation of resveratrol and polydatin, albeit with low activity. Importantly, CYP736A61 mediated the cleavage of resveratrol and polydatin as well as pinostilbene and pterostilbene. Interestingly, CY736A61 also converted phloretin to naringenin chalcone. These results suggest that CYP736A61 is a novel CYP enzyme with stilbene cleavage activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estilbenos / Solanum lycopersicum / Glucósidos Idioma: En Revista: Enzyme Microb Technol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estilbenos / Solanum lycopersicum / Glucósidos Idioma: En Revista: Enzyme Microb Technol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos