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Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.
Nigishi, Minori; Shimakawa, Ginga; Yamagishi, Kansei; Amano, Ryosuke; Ito, Shun; Tsuji, Yoshinori; Nagasato, Chikako; Matsuda, Yusuke.
Afiliación
  • Nigishi M; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Shimakawa G; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Yamagishi K; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Amano R; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Ito S; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Tsuji Y; Department of Bioscience, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo 669-1330, Japan.
  • Nagasato C; Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
  • Matsuda Y; Field Science Center for Northern Biosphere, Muroran Marine Station, Hokkaido University, Muroran 051-0013, Japan.
Plant Physiol ; 195(2): 1432-1445, 2024 May 31.
Article en En | MEDLINE | ID: mdl-38478576
ABSTRACT
Anion transporters sustain a variety of physiological states in cells. Bestrophins (BSTs) belong to a Cl- and/or HCO3- transporter family conserved in bacteria, animals, algae, and plants. Recently, putative BSTs were found in the green alga Chlamydomonas reinhardtii, where they are upregulated under low CO2 (LC) conditions and play an essential role in the CO2-concentrating mechanism (CCM). The putative BST orthologs are also conserved in diatoms, secondary endosymbiotic algae harboring red-type plastids, but their physiological functions are unknown. Here, we characterized the subcellular localization and expression profile of BSTs in the marine diatoms Phaeodactylum tricornutum (PtBST1 to 4) and Thalassiosira pseudonana (TpBST1 and 2). PtBST1, PtBST2, and PtBST4 were localized at the stroma thylakoid membrane outside of the pyrenoid, and PtBST3 was localized in the pyrenoid. Contrarily, TpBST1 and TpBST2 were both localized in the pyrenoid. These BST proteins accumulated in cells grown in LC but not in 1% CO2 (high CO2 [HC]). To assess the physiological functions, we generated knockout mutants for the PtBST1 gene by genome editing. The lack of PtBST1 decreased photosynthetic affinity for dissolved inorganic carbon to the level comparable with the HC-grown wild type. Furthermore, non-photochemical quenching in LC-grown cells was 1.5 to 2.0 times higher in the mutants than in the wild type. These data suggest that HCO3- transport at the stroma thylakoid membranes by PtBST1 is a critical part of the CO2-evolving machinery of the pyrenoid in the fully induced CCM and that PtBST1 may modulate photoprotection under CO2-limited environments in P. tricornutum.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotosíntesis / Dióxido de Carbono / Diatomeas Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotosíntesis / Dióxido de Carbono / Diatomeas Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos