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Group C MAP kinases phosphorylate MBD10 to regulate ABA-induced leaf senescence in Arabidopsis.
Li, Yangdan; Kamiyama, Yoshiaki; Minegishi, Fuko; Tamura, Yuki; Yamashita, Kota; Katagiri, Sotaro; Takase, Hinano; Otani, Masahiko; Tojo, Ryo; Rupp, Gabrielle E; Suzuki, Takamasa; Kawakami, Naoto; Peck, Scott C; Umezawa, Taishi.
Afiliación
  • Li Y; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Kamiyama Y; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Minegishi F; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Tamura Y; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Yamashita K; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Katagiri S; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Takase H; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
  • Otani M; School of Agriculture, Meiji University, Kawasaki, 214-8571, Kanagawa, Japan.
  • Tojo R; School of Agriculture, Meiji University, Kawasaki, 214-8571, Kanagawa, Japan.
  • Rupp GE; Department of Biochemistry, University of Missouri, Columbia, 65211, Missouri, USA.
  • Suzuki T; College of Bioscience and Biotechnology, Chubu University, Kasugai, 487-8501, Aichi, Japan.
  • Kawakami N; School of Agriculture, Meiji University, Kawasaki, 214-8571, Kanagawa, Japan.
  • Peck SC; Department of Biochemistry, University of Missouri, Columbia, 65211, Missouri, USA.
  • Umezawa T; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan.
Plant J ; 118(6): 1747-1759, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38477703
ABSTRACT
Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG-binding domain 10 (MBD10) as a phosphoprotein that becomes differentially phosphorylated after ABA treatment in Arabidopsis. ABA-induced leaf senescence was delayed in mbd10 knockout plants but accelerated in MBD10-overexpressing plants, suggesting that MBD10 positively regulates ABA-induced leaf senescence. ABA-induced phosphorylation of MBD10 occurs in planta on Thr-89, and our results demonstrated that Thr-89 phosphorylation is essential for MBD10's function in leaf senescence. The in vivo phosphorylation of Thr-89 in MBD10 was significantly downregulated in a quadruple mutant of group C MAPKs (mpk1/2/7/14), and group C MAPKs directly phosphorylated MBD10 in vitro. Furthermore, mpk1/2/7/14 showed a similar phenotype as seen in mbd10 for ABA-induced leaf senescence, suggesting that group C MAPKs are the cognate kinases of MBD10 for Thr-89. Because group C MAPKs have been reported to function downstream of SnRK2s, our results indicate that group C MAPKs and MBD10 constitute a regulatory pathway for ABA-induced leaf senescence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Hojas de la Planta / Proteínas Quinasas Activadas por Mitógenos / Proteínas de Arabidopsis / Senescencia de la Planta Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Hojas de la Planta / Proteínas Quinasas Activadas por Mitógenos / Proteínas de Arabidopsis / Senescencia de la Planta Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido