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The Role of ß-Dystroglycan in Nuclear Dynamics.
Cook, Matthew; Stevenson, Ben; Jacobs, Laura A; Leocadio Victoria, Daniel; Cisneros, Bulmaro; Hobbs, Jamie K; Stewart, Colin L; Winder, Steve J.
Afiliación
  • Cook M; School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.
  • Stevenson B; A*STAR Skin Research Laboratories, Singapore 138648, Singapore.
  • Jacobs LA; School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.
  • Leocadio Victoria D; School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.
  • Cisneros B; School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.
  • Hobbs JK; Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados, Mexico City 07360, Mexico.
  • Stewart CL; Department of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, UK.
  • Winder SJ; A*STAR Skin Research Laboratories, Singapore 138648, Singapore.
Cells ; 13(5)2024 Feb 29.
Article en En | MEDLINE | ID: mdl-38474395
ABSTRACT
Dystroglycan is a ubiquitously expressed heterodimeric cell-surface laminin receptor with roles in cell adhesion, signalling, and membrane stabilisation. More recently, the transmembrane ß-subunit of dystroglycan has been shown to localise to both the nuclear envelope and the nucleoplasm. This has led to the hypothesis that dystroglycan may have a structural role at the nuclear envelope analogous to its role at the plasma membrane. The biochemical fraction of myoblast cells clearly supports the presence of dystroglycan in the nucleus. Deletion of the dystroglycan protein by disruption of the DAG1 locus using CRISPR/Cas9 leads to changes in nuclear size but not overall morphology; moreover, the Young's modulus of dystroglycan-deleted nuclei, as determined by atomic force microscopy, is unaltered. Dystroglycan-disrupted myoblasts are also no more susceptible to nuclear stresses including chemical and mechanical, than normal myoblasts. Re-expression of dystroglycan in DAG1-disrupted myoblasts restores nuclear size without affecting other nuclear parameters.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Laminina / Distroglicanos Idioma: En Revista: Cells Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Laminina / Distroglicanos Idioma: En Revista: Cells Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza