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OsOFP6 Overexpression Alters Plant Architecture, Grain Shape, and Seed Fertility.
Zhu, Xuting; Li, Yuan; Zhao, Xiangqian; Feng, Yukai; Bao, Zhengkai; Liu, Wenzhen; Li, Feifei.
Afiliación
  • Zhu X; College of Advanced Agricultural Science, Zhejiang A&F University, Hangzhou 311300, China.
  • Li Y; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Zhao X; College of Advanced Agricultural Science, Zhejiang A&F University, Hangzhou 311300, China.
  • Feng Y; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
  • Bao Z; College of Advanced Agricultural Science, Zhejiang A&F University, Hangzhou 311300, China.
  • Liu W; College of Advanced Agricultural Science, Zhejiang A&F University, Hangzhou 311300, China.
  • Li F; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Int J Mol Sci ; 25(5)2024 Mar 01.
Article en En | MEDLINE | ID: mdl-38474136
ABSTRACT
OVATE family proteins (OFPs) play important roles in plant growth and development, hormone signaling, and stress response pathways. However, the functions of OsOFPs in rice are largely unknown. In this study, a novel gain-of-function rice mutant, Osofp6-D, was identified. This mutant exhibited decreased plant height, erect leaves, reduced panicle size, short and wide seeds, delayed seed germination time, and reduced fertility. These phenotypic changes were attributed to the increased expression of OsOFP6, which was caused by a T-DNA insertion. Complementation of the Osofp6-D phenotype by knockout of OsOFP6 using the CRISPR/Cas9 system confirmed that the Osofp6-D phenotype was caused by OsOFP6 overexpression. In addition, transgenic plants overexpressing OsOFP6 with the 35S promoter mimicked the Osofp6-D phenotype. Cytological observations of the glumes showed that OsOFP6 overexpression altered the grain shape, mainly by altering the cell shape. Hormone response experiments showed that OsOFP6 was involved in the gibberellin (GA) and brassinolide (BR) signaling responses. Further studies revealed that OsOFP6 interacts with E3BB, which is orthologous to the Arabidopsis central organ size-control protein BIG BROTHER (BB). This study further elucidates the regulation mechanism of the rice OFP family on plant architecture and grain shape.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Arabidopsis Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Arabidopsis Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza