Your browser doesn't support javascript.
loading
Evaluation of the cytotoxic activity of sorafenib-loaded camel milk casein nanoparticles against hepatocarcinoma cells.
Mittal, Aastha; Mahala, Neelam; Dhanawade, Nikhil Hanamant; Dubey, Sunil Kumar; Dubey, Uma S.
Afiliación
  • Mittal A; Department of Biological Sciences, Birla Institute of Technology of Science (BITS), Pilani Campus, Pilani, Rajasthan, India.
  • Mahala N; Department of Biological Sciences, Birla Institute of Technology of Science (BITS), Pilani Campus, Pilani, Rajasthan, India.
  • Dhanawade NH; Department of Biotechnology, Parul Institute of Technology, Parul University, Vadodara, Gujarat, India.
  • Dubey SK; Department of Biological Sciences, Birla Institute of Technology of Science (BITS), Pilani Campus, Pilani, Rajasthan, India.
  • Dubey US; R & D Healthcare Division Emami Ltd, Kolkata, India.
Biotechnol J ; 19(3): e2300449, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38472095
ABSTRACT
Sorafenib, a multikinase inhibitor is used to treat hepatocellular and renal carcinoma. However, a low solubility impedes its bioavailability and thus, effectiveness. This study aims to enhance its effectiveness by using novel camel milk casein nanoparticles as a delivery system. This study evaluates the cytotoxicity of sorafenib encapsulated in camel milk casein nanoparticles against human hepatocarcinoma cells (HepG2 cells) in vitro. Optimal drug loaded nanoparticles were stable for 1 month, had encapsulation efficiency of 96%, exhibited a particle size of 230 nm, zeta potential of -14.4 and poly disparity index of 0.261. Treatment with it led to cell morphology and DNA fragmentation as a characteristic of apoptosis. Flow cytometry showed G1 phase arrest of cell cycle and 26% increased apoptotic cells population upon treatment as compared to control. Sorafenib-loaded casein nanoparticles showed 6-fold increased ROS production in HepG2 cells as compared to 4-fold increase shown by the free drug. Gene and protein expression studies done by qPCR and western blotting depicted upregulation of tumor suppressor gene p53, pro-apoptotic Bax, and caspase-3 along with downregulated anti-apoptotic Bcl-2 gene and protein expression which further emphasized death by apoptosis. It is concluded regarding the feasibility of these casein nanoparticles as a delivery system with enhanced therapeutic outcomes against hepatocellular carcinoma cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Nanopartículas / Neoplasias Hepáticas / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Biotechnol J Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Nanopartículas / Neoplasias Hepáticas / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Biotechnol J Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania