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Virulence genes, antimicrobial resistance profile, phylotyping and pathotyping of diarrheagenic Escherichia coli isolated from children in Southwest Mexico.
Tapia-Pastrana, Gabriela; Rojas-Bautista, Metztli; Hernández-Pérez, Pilar; Santiago-Martínez, Olegario; Gómez-Rodríguez, Lucía C; Terrazas-Luna, Víctor M; Montes-Yedra, Jacobo; Bautista-Avendaño, Alfonso A; García-López, Eduardo S; Leon-Sicairos, Nidia; Angulo-Zamudio, Uriel A; Canizalez-Roman, Adrian.
Afiliación
  • Tapia-Pastrana G; Laboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, Servicios de Salud, IMSS-Bienestar, Oaxaca, Mexico.
  • Rojas-Bautista M; Facultad de Ciencias Químicas de la Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juarez, Oaxaca, Mexico.
  • Hernández-Pérez P; Facultad de Ciencias Químicas de la Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juarez, Oaxaca, Mexico.
  • Santiago-Martínez O; Facultad de Ciencias Químicas de la Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juarez, Oaxaca, Mexico.
  • Gómez-Rodríguez LC; Facultad de Ciencias Químicas de la Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juarez, Oaxaca, Mexico.
  • Terrazas-Luna VM; Laboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, Servicios de Salud, IMSS-Bienestar, Oaxaca, Mexico.
  • Montes-Yedra J; Departamento de Ciencias Básicas del Instituto Tecnológico del Valle de Oaxaca, Santa Cruz Xoxocotlán, Oaxaca, Mexico.
  • Bautista-Avendaño AA; Departamento de Ciencias Básicas del Instituto Tecnológico del Valle de Oaxaca, Santa Cruz Xoxocotlán, Oaxaca, Mexico.
  • García-López ES; Laboratorio Estatal de Salud Publica Oaxaca, Reyes Mantecón, Oaxaca, Mexico.
  • Leon-Sicairos N; School of Medicine, Autonomous University of Sinaloa, Culiacan, Sinaloa, Mexico.
  • Angulo-Zamudio UA; Pediatric Hospital of Sinaloa, Culiacan, Sinaloa, Mexico.
  • Canizalez-Roman A; School of Medicine, Autonomous University of Sinaloa, Culiacan, Sinaloa, Mexico.
PLoS One ; 19(3): e0300304, 2024.
Article en En | MEDLINE | ID: mdl-38470897
ABSTRACT
Diarrheagenic E. coli (DEC) strains are one of the most important etiology factors causing diarrhea in children worldwide, especially in developing countries. DEC strains have characteristic virulence factors; however, other supplemental virulence genes (SVG) may contribute to the development of diarrhea in children. Therefore, this study aimed to determine the prevalence of DEC in children with diarrhea in southwestern Mexico and to associate childhood symptoms, SVG, and pathotypes with diarrhea-causing DEC strains. DEC strains were isolated from 230 children with diarrhea aged 0-60 months from the state of Oaxaca, southwestern Mexico; clinical data were collected, and PCR was used to identify SVG and pathotypes. Antibiotic resistance profiling was performed on DEC strains. 63% of samples were DEC positive, single or combined infections (two (21%) or three strains (1.3%)) of aEPEC (51%), EAEC (10.2%), tEPEC (5.4%), DAEC (4.8%), ETEC (4.1%), EIEC (1.4%), or EHEC (0.7%) were found. Children aged ≤ 12 and 49-60 months and symptoms (e.g., fever and blood) were associated with DEC strains. SVG related to colonization (nleB-EHEC), cytotoxicity (sat-DAEC and espC-tEPEC), and proteolysis (pic-aEPEC) were associated with DECs strains. E. coli phylogroup A was the most frequent, and some pathotypes (aEPEC-A, DAEC-B), and SVG (espC-B2, and sat-D) were associated with the phylogroups. Over 79% of the DEC strains were resistant to antibiotics, and 40% were MDR and XDR, respectively. In conclusion aEPEC was the most prevalent pathotype in children with diarrhea in this region. SVG related to colonization, cytotoxicity, and proteolysis were associated with diarrhea-producing DEC strains, which may play an essential role in the development of diarrhea in children in southwestern Mexico.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Infecciones por Escherichia coli Límite: Child / Humans País/Región como asunto: Mexico Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Infecciones por Escherichia coli Límite: Child / Humans País/Región como asunto: Mexico Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: México Pais de publicación: Estados Unidos