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Role of the GLP2-Wnt1 axis in silicon-rich alkaline mineral water maintaining intestinal epithelium regeneration in piglets under early-life stress.
Chen, Jian; Dai, Xue-Yan; Zhao, Bi-Chen; Xu, Xiang-Wen; Kang, Jian-Xun; Xu, Ya-Ru; Li, Jin-Long.
Afiliación
  • Chen J; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Dai XY; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Zhao BC; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Xu XW; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Kang JX; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Xu YR; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
  • Li JL; College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. Jinlongli@neau.edu.cn.
Cell Mol Life Sci ; 81(1): 126, 2024 Mar 12.
Article en En | MEDLINE | ID: mdl-38470510
ABSTRACT
Stress-induced intestinal epithelial injury (IEI) and a delay in repair in infancy are predisposing factors for refractory gut diseases in adulthood, such as irritable bowel syndrome (IBS). Hence, it is necessary to develop appropriate mitigation methods for mammals when experiencing early-life stress (ELS). Weaning, as we all know, is a vital procedure that all mammalian newborns, including humans, must go through. Maternal separation (MS) stress in infancy (regarded as weaning stress in animal science) is a commonly used ELS paradigm. Drinking silicon-rich alkaline mineral water (AMW) has a therapeutic effect on enteric disease, but the specific mechanisms involved have not been reported. Herein, we discover the molecular mechanism by which silicon-rich AMW repairs ELS-induced IEI by maintaining intestinal stem cell (ISC) proliferation and differentiation through the glucagon-like peptide (GLP)2-Wnt1 axis. Mechanistic study showed that silicon-rich AMW activates GLP2-dependent Wnt1/ß-catenin pathway, and drives ISC proliferation and differentiation by stimulating Lgr5+ ISC cell cycle passage through the G1-S-phase checkpoint, thereby maintaining intestinal epithelial regeneration and IEI repair. Using GLP2 antagonists (GLP23-33) and small interfering RNA (SiWnt1) in vitro, we found that the GLP2-Wnt1 axis is the target of silicon-rich AMW to promote intestinal epithelium regeneration. Therefore, silicon-rich AMW maintains intestinal epithelium regeneration through the GLP2-Wnt1 axis in piglets under ELS. Our research contributes to understanding the mechanism of silicon-rich AMW promoting gut epithelial regeneration and provides a new strategy for the alleviation of ELS-induced IEI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Experiencias Adversas de la Infancia / Aguas Minerales Límite: Animals / Humans / Newborn Idioma: En Revista: Cell Mol Life Sci Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Experiencias Adversas de la Infancia / Aguas Minerales Límite: Animals / Humans / Newborn Idioma: En Revista: Cell Mol Life Sci Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Suiza