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The role of the 5' sensing function of ribonuclease E in cyanobacteria.
Hoffmann, Ute A; Lichtenberg, Elisabeth; Rogh, Said N; Bilger, Raphael; Reimann, Viktoria; Heyl, Florian; Backofen, Rolf; Steglich, Claudia; Hess, Wolfgang R; Wilde, Annegret.
Afiliación
  • Hoffmann UA; Molecular Genetics of Prokaryotes, Institute of Biology III, University of Freiburg, Freiburg, Germany.
  • Lichtenberg E; School of Engineering Sciences in Chemistry, Biotechnology and Health, Science for Life Laboratory, KTH - Royal Institute of Technology, Stockholm, Sweden.
  • Rogh SN; Molecular Genetics of Prokaryotes, Institute of Biology III, University of Freiburg, Freiburg, Germany.
  • Bilger R; Molecular Genetics of Prokaryotes, Institute of Biology III, University of Freiburg, Freiburg, Germany.
  • Reimann V; Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Freiburg, Germany.
  • Heyl F; Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Freiburg, Germany.
  • Backofen R; Bioinformatics Group, Department of Computer Science, University of Freiburg, Freiburg, Germany.
  • Steglich C; Bioinformatics Group, Department of Computer Science, University of Freiburg, Freiburg, Germany.
  • Hess WR; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.
  • Wilde A; Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Freiburg, Germany.
RNA Biol ; 21(1): 1-18, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38469716
ABSTRACT
RNA degradation is critical for synchronising gene expression with changing conditions in prokaryotic and eukaryotic organisms. In bacteria, the preference of the central ribonucleases RNase E, RNase J and RNase Y for 5'-monophosphorylated RNAs is considered important for RNA degradation. For RNase E, the underlying mechanism is termed 5' sensing, contrasting to the alternative 'direct entry' mode, which is independent of monophosphorylated 5' ends. Cyanobacteria, such as Synechocystis sp. PCC 6803 (Synechocystis), encode RNase E and RNase J homologues. Here, we constructed a Synechocystis strain lacking the 5' sensing function of RNase E and mapped on a transcriptome-wide level 283 5'-sensing-dependent cleavage sites. These included so far unknown targets such as mRNAs encoding proteins related to energy metabolism and carbon fixation. The 5' sensing function of cyanobacterial RNase E is important for the maturation of rRNA and several tRNAs, including tRNAGluUUC. This tRNA activates glutamate for tetrapyrrole biosynthesis in plant chloroplasts and in most prokaryotes. Furthermore, we found that increased RNase activities lead to a higher copy number of the major Synechocystis plasmids pSYSA and pSYSM. These results provide a first step towards understanding the importance of the different target mechanisms of RNase E outside Escherichia coli.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Synechocystis / Endorribonucleasas Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Synechocystis / Endorribonucleasas Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos