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Genomic and transcriptomic characterization of methylmercury detoxification in a deep ocean Alteromonas mediterranea ISS312.
Pereira-Garcia, Carla; Sanz-Sáez, Isabel; Sánchez, Pablo; Coutinho, Felipe H; Bravo, Andrea G; Sánchez, Olga; Acinas, Silvia G.
Afiliación
  • Pereira-Garcia C; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain; Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Catalunya, Spain. Electronic
  • Sanz-Sáez I; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain; Department of Environmental Chemistry, IDAEA-CSIC, Barcelona, Catalunya, Spain.
  • Sánchez P; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain.
  • Coutinho FH; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain.
  • Bravo AG; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain.
  • Sánchez O; Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Catalunya, Spain.
  • Acinas SG; Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain. Electronic address: sacinas@icm.csic.es.
Environ Pollut ; 347: 123725, 2024 Apr 15.
Article en En | MEDLINE | ID: mdl-38467369
ABSTRACT
Methylmercury (MeHg) is one of the most worrisome pollutants in marine systems. MeHg detoxification is mediated by merB and merA genes, responsible for the demethylation of MeHg and the reduction of inorganic mercury, respectively. Little is known about the biological capacity to detoxify this compound in marine environments, and even less the bacterial transcriptional changes during MeHg detoxification. This study provides the genomic and transcriptomic characterization of the deep ocean bacteria Alteromonas mediterranea ISS312 with capacity for MeHg degradation. Its genome sequence revealed four mer operons containing three merA gene and two merB gene copies, that could be horizontally transferred among distant related genomes by mobile genetic elements. The transcriptomic profiling in the presence of 5 µM MeHg showed that merA and merB genes are within the most expressed genes, although not all mer genes were equally transcribed. Besides, we aimed to identify functional orthologous genes that displayed expression profiles highly similar or identical to those genes within the mer operons, which could indicate they are under the same regulatory controls. We found contrasting expression profiles for each mer operon that were positively correlated with a wide array of functions mostly related to amino acid metabolism, but also to flagellar assembly or two component systems. Also, this study highlights that all merAB genes of the four operons were globally distributed across oceans layers with higher transcriptional activity in the mesopelagic deeper waters. Our study provides new insights about the transcriptional patterns related to the capacity of marine bacteria to detoxify MeHg, with important implications for the understanding of this process in marine ecosystems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alteromonas / Mercurio / Compuestos de Metilmercurio Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alteromonas / Mercurio / Compuestos de Metilmercurio Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido