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Diagnosis of Mycobacterium tuberculosis using palladium-platinum bimetallic nanoparticles combined with paper-based analytical devices.
Tung, Cheng-Yang; Tsai, Tsung-Ting; Chiu, Ping-Yeh; Viter, Roman; Ramanavicius, Arunas; Yu, Cheng-Ju; Chen, Chien-Fu.
Afiliación
  • Tung CY; Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan. stevechen@ntu.edu.tw.
  • Tsai TT; Department of Orthopaedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
  • Chiu PY; Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan. stevechen@ntu.edu.tw.
  • Viter R; Department of Orthopaedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
  • Ramanavicius A; Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas Street 3, LV-1004 Riga, Latvia.
  • Yu CJ; State Research Institute Center for Physical and Technological Sciences, Sauletekio Ave. 3, LT-10257 Vilnius, Lithuania.
  • Chen CF; Department of Applied Physics and Chemistry, University of Taipei, Taipei 100, Taiwan. cheng-ju@utaipei.edu.tw.
Nanoscale ; 16(12): 5988-5998, 2024 Mar 21.
Article en En | MEDLINE | ID: mdl-38465745
ABSTRACT
In this study, we demonstrate that palladium-platinum bimetallic nanoparticles (Pd@Pt NPs) as the nanozyme, combined with a multi-layer paper-based analytical device and DNA hybridization, can successfully detect Mycobacterium tuberculosis. This nanozyme has peroxidase-like properties, which can increase the oxidation rate of the substrate. Compared with horseradish peroxidase, which is widely used in traditional detection, the Michaelis constants of Pd@Pt NPs are fourteen and seventeen times lower than those for 3,3',5,5'-tetramethylbenzidine and H2O2, respectively. To verify the catalytic efficiency of Pd@Pt NPs, this study will execute molecular diagnosis of Mycobacterium tuberculosis. We chose the IS6110 fragment as the target DNA and divided the complementary sequences into the capture DNA and reporter DNA. They were modified on paper and Pd@Pt NPs, respectively, to detect Mycobacterium tuberculosis on a paper-based analytical device. With the above-mentioned method, we can detect target DNA within 15 minutes with a linear range between 0.75 and 10 nM, and a detection limit of 0.216 nM. These results demonstrate that the proposed platform (a DNA-nanozyme integrated paper-based analytical device, dnPAD) can provide sensitive and on-site infection prognosis in areas with insufficient medical resources.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Mycobacterium tuberculosis Idioma: En Revista: Nanoscale Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Mycobacterium tuberculosis Idioma: En Revista: Nanoscale Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido