Your browser doesn't support javascript.
loading
Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals.
Choi, Gyuho; Kang, Hyunkoo; Suh, Jung-Soo; Lee, Haksoo; Han, Kiseok; Yoo, Gaeun; Jo, Hyejin; Shin, Yeong Min; Kim, Tae-Jin; Youn, BuHyun.
Afiliación
  • Choi G; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Kang H; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Suh JS; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Lee H; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Han K; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Yoo G; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Jo H; Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Republic of Korea.
  • Shin YM; Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Republic of Korea.
  • Kim TJ; Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea.
  • Youn B; Department of Biological Sciences, Pusan National University, Busan 46241, Republic of Korea.
Biomater Res ; 28: 0010, 2024.
Article en En | MEDLINE | ID: mdl-38464469
ABSTRACT
The increasing prevalence of endocrine-disrupting chemicals (EDCs) in our environment is a growing concern, with numerous studies highlighting their adverse effects on the human endocrine system. Among the EDCs, estrogenic endocrine-disrupting chemicals (eEDCs) are exogenous compounds that perturb estrogenic hormone function by interfering with estrogen receptor (ER) homo (α/α, ß/ß) or hetero (α/ß) dimerization. To date, a comprehensive screening approach for eEDCs affecting all ER dimer forms in live cells is lacking. Here, we developed ER dimerization-detecting biosensors (ERDDBs), based on bioluminescence resonance energy transfer, for dimerization detection and rapid eEDC identification. To enhance the performance of these biosensors, we determined optimal donor and acceptor locations using computational analysis. Additionally, employing HaloTag as the acceptor and incorporating the P2A peptide as a linker yielded the highest sensitivity among the prototypes. We also established stable cell lines to screen potential ER dimerization inducers among estrogen analogs (EAs). The EAs were categorized through cross-comparison of ER dimer responses, utilizing EC values derived from a standard curve established with 17ß-estradiol. We successfully classified 26 of 72 EAs, identifying which ER dimerization types they induce. Overall, our study underscores the effectiveness of the optimized ERDDB for detecting ER dimerization and its applicability in screening and identifying eEDCs.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomater Res Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomater Res Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos