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Mitochondrial DNA levels in perfusate and bile during ex vivo normothermic machine correspond with donor liver quality.
Westhaver, Lauren P; Nersesian, Sarah; Arseneau, Riley J; Hefler, Joshua; Hargreaves, Breanna K V; Edgar, Alexander; Azizieh, Yara; Cuesta-Gomez, Nerea; Izquierdo, Dayne L; Shapiro, A M James; Gala-Lopez, Boris L; Boudreau, Jeanette E.
Afiliación
  • Westhaver LP; Department of Pathology, Dalhousie University, Halifax, NS, Canada.
  • Nersesian S; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Arseneau RJ; Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
  • Hefler J; Department of Pathology, Dalhousie University, Halifax, NS, Canada.
  • Hargreaves BKV; Department of Surgery, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
  • Edgar A; Dalhousie Medical School NS, Dalhousie University, Halifax, NS, Canada.
  • Azizieh Y; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Cuesta-Gomez N; Department of Pathology, Dalhousie University, Halifax, NS, Canada.
  • Izquierdo DL; Department of Surgery, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
  • Shapiro AMJ; Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada.
  • Gala-Lopez BL; Department of Surgery, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
  • Boudreau JE; Department of Surgery, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada.
Heliyon ; 10(5): e27122, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38463874
ABSTRACT
Ex vivo normothermic machine perfusion (NMP) preserves donor organs and permits real-time assessment of allograft health, but the most effective indicators of graft viability are uncertain. Mitochondrial DNA (mtDNA), released consequent to traumatic cell injury and death, including the ischemia-reperfusion injury inherent in transplantation, may meet the need for a biomarker in this context. We describe a real time PCR-based approach to assess cell-free mtDNA during NMP as a universal biomarker of allograft quality. Measured in the perfusate fluid of 29 livers, the quantity of mtDNA correlated with metrics of donor liver health including International Normalized Ratio (INR), lactate, and warm ischemia time, and inversely correlated with inferior vena cava (IVC) flow during perfusion. Our findings endorse mtDNA as a simple and rapidly measured feature that can inform donor liver health, opening the possibility to better assess livers acquired from extended criteria donors to improve organ supply.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido