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Metformin plus L-carnitine enhances brown/beige adipose tissue activity via Nrf2/HO-1 signaling to reduce lipid accumulation and inflammation in murine obesity.
Liang, Guojin; Fang, Jie; Zhang, Pingping; Ding, Shuxia; Zhao, Yudan; Feng, Yueying.
Afiliación
  • Liang G; Anesthesiology Department, Ningbo First Hospital, Ningbo, China.
  • Fang J; Paediatrics Department, Ningbo Women and Children's Hospital, Zhejiang, 315000, China.
  • Zhang P; Paediatrics Department, Ningbo Women and Children's Hospital, Zhejiang, 315000, China.
  • Ding S; Paediatrics Department, Ningbo Women and Children's Hospital, Zhejiang, 315000, China.
  • Zhao Y; Paediatrics Department, Ningbo Women and Children's Hospital, Zhejiang, 315000, China.
  • Feng Y; Paediatrics Department, Ningbo Women and Children's Hospital, No. 339 Liuting Street, Ningbo, Zhejiang, 315000, China.
Open Med (Wars) ; 19(1): 20240900, 2024.
Article en En | MEDLINE | ID: mdl-38463531
ABSTRACT
This study investigated how Metformin (Met) combined with L-carnitine (L-car) modulates brown adipose tissue (BAT) to affect obesity. High-fat-induced obese rats received daily oral gavage with Met and/or L-car, followed by serum biochemical analysis, histopathological observation on adipose tissues, and immunochemistry test for the abdominal expression of BAT-specific uncoupling protein 1 (UCP1). Mouse-embryonic-fibroblast cells were induced into adipocytes, during which Met plus L-car was added with/without saturated fatty acid (SFA). The role of nuclear factor erythroid 2-related factor 2 (Nrf2) in adipocyte browning was investigated by gene silencing. Mitochondria biogenesis in adipocytes was inspected by Mitotracker staining. Nrf2/heme oxygenase-1 (HO-1)/BAT-related genes/proinflammatory marker expressions in adipose tissues and/or adipocytes were analyzed by Western blot, qRT-PCR, and/or immunofluorescence test. Met or L-car improved metabolic disorders, reduced adipocyte vacuolization and swelling, upregulated levels of BAT-related genes including UCP1 and downregulated proinflammatory marker expressions, and activated the Nrf2/HO-1 pathway in adipose tissues of obese rats. Met and L-car functioned more strongly than alone. In adipocytes, Met plus L-car upregulated BAT-related gene levels and protected against SFA-caused inflammation promotion and mitochondria degeneration, which yet was attenuated by Nrf2 silencing. Met plus L-car enhances BAT activity and white adipose tissue browning via the Nrf2/HO-1 pathway to reduce lipid accumulation and inflammation in obese rats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Med (Wars) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Med (Wars) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia