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Exosomal circRNAs in the plasma serve as novel biomarkers for IPF diagnosis and progression prediction.
Gan, Wenhua; Song, Wenwen; Gao, Yujuan; Zheng, Xuexue; Wang, Fengjuan; Zhang, Zirui; Zen, Ke; Liang, Hongwei; Yan, Xin.
Afiliación
  • Gan W; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
  • Song W; Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of China Pharmaceutical University, Nanjing, 210008, China.
  • Gao Y; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
  • Zheng X; Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of China Pharmaceutical University, Nanjing, 210008, China.
  • Wang F; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
  • Zhang Z; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
  • Zen K; Department of Thoracic Surgery, Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, 210008, China.
  • Liang H; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China. kzen@nju.edu.cn.
  • Yan X; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China. hwliang@cpu.edu.cn.
J Transl Med ; 22(1): 264, 2024 03 10.
Article en En | MEDLINE | ID: mdl-38462601
ABSTRACT

BACKGROUND:

Idiopathic Pulmonary Fibrosis (IPF) is a type of chronic interstitial pneumonia, often fatal, with elusive causes and a bleak prognosis. Its treatment options are limited and largely ineffective. Early detection and precise diagnosis are pivotal in managing the disease effectively and enhancing patient survival rates. Recently, the quest for trustworthy biomarkers for IPF has gained momentum. Notably, emerging studies indicate that circular RNAs (circRNAs) found in exosomes may hold significant potential as valuable diagnostic markers.

METHODS:

In this study, we initially explored the expression profile of circRNAs in exosomes sourced from the blood of IPF patients and healthy volunteers, employing a human circRNA microarray. We then utilized RT-qPCR to corroborate the dysregulated circRNAs identified by the microarray during the training phase. Next, the circRNAs that displayed a significant increase during the training phase were selected for further validation in a larger cohort encompassing 113 IPF patients and 76 healthy volunteers. Ultimately, the expression level and function of hsa_circ_0044226 were substantiated through a series of in vivo and in vitro experiments.

RESULTS:

Utilizing a human circRNA microarray, we identified 11 dysregulated circRNAs in the exosomes derived from the blood of IPF patients and control volunteers. Subsequent RT-qPCR analysis revealed significant increases in three circRNAs (hsa_circ_0044226, hsa_circ_0004099, hsa_circ_0008898) within the IPF patients. Notably, hsa_circ_0044226 was markedly elevated in patients experiencing acute exacerbation of IPF (AE-IPF) compared to those with stable IPF (S-IPF). Additionally, an upregulation of hsa_circ_0044226 was observed in the blood exosomes derived from a bleomycin-induced IPF mouse model.

CONCLUSION:

The expression levels of hsa_circ_0044226, hsa_circ_0004099, and hsa_circ_0008898 in plasma exosomes introduce a new paradigm of biomarkers for the diagnosis and progression of IPF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar Idiopática / ARN Circular Límite: Animals / Humans Idioma: En Revista: J Transl Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibrosis Pulmonar Idiopática / ARN Circular Límite: Animals / Humans Idioma: En Revista: J Transl Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido