Control of poly(A)-tail length and translation in vertebrate oocytes and early embryos.

Xiang, Kehui; Ly, Jimmy; Bartel, David P

Xiang, Kehui; Ly, Jimmy; Bartel, David P.

Afiliación

Xiang K; Howard Hughes Medical Institute, Cambridge, MA 02142, USA; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Ly J; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Bartel DP; Howard Hughes Medical Institute, Cambridge, MA 02142, USA; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: dbartel@wi.mit.edu.

Dev Cell ; 59(8): 1058-1074.e11, 2024 Apr 22.

Article en En | MEDLINE | ID: mdl-38460509

ABSTRACT

ABSTRACT

During oocyte maturation and early embryogenesis, changes in mRNA poly(A)-tail lengths strongly influence translation, but how these tail-length changes are orchestrated has been unclear. Here, we performed tail-length and translational profiling of mRNA reporter libraries (each with millions of 3' UTR sequence variants) in frog oocytes and embryos and in fish embryos. Contrasting to previously proposed cytoplasmic polyadenylation elements (CPEs), we found that a shorter element, UUUUA, together with the polyadenylation signal (PAS), specify cytoplasmic polyadenylation, and we identified contextual features that modulate the activity of both elements. In maturing oocytes, this tail lengthening occurs against a backdrop of global deadenylation and the action of C-rich elements that specify tail-length-independent translational repression. In embryos, cytoplasmic polyadenylation becomes more permissive, and additional elements specify waves of stage-specific deadenylation. Together, these findings largely explain the complex tapestry of tail-length changes observed in early frog and fish development, with strong evidence of conservation in both mice and humans.

Asunto(s)

Regiones no Traducidas 3'; Oocitos; Poli A; Poliadenilación; Biosíntesis de Proteínas; ARN Mensajero; Animales; Oocitos/metabolismo; Oocitos/citología; Poli A/metabolismo; Poli A/genética; Regiones no Traducidas 3'/genética; ARN Mensajero/genética; ARN Mensajero/metabolismo; Regulación del Desarrollo de la Expresión Génica; Ratones; Humanos; Embrión no Mamífero/metabolismo; Desarrollo Embrionario/genética; Femenino; Xenopus laevis/metabolismo; Xenopus laevis/embriología; Xenopus laevis/genética; Citoplasma/metabolismo

Palabras clave

3' UTR; CPE; CPEB; cytoplasmic polyadenylation; deadenylation; embryonic development; gene regulation; oocyte maturation; poly(A) tails; translation regulation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Poli A / Biosíntesis de Proteínas / ARN Mensajero / Regiones no Traducidas 3' / Poliadenilación Límite: Animals / Female / Humans Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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