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Fertilization with follicular fluid reduces HSP70 and BAX expression on bovine in vitro embryos.
Homobono, Bruno Porpino; das Mercês, Marcela Oliveira; Nogueira, Leonardo Henrique da Silva; de Souza, Eduardo Baia; Cardoso, Ana Paloma Lobo; Santos, Ana Beatriz Sousa; Ramos, Anelise de Sarges; Costa, Matheus Hadigo Pires; Santana, Priscila Di Paula Bessa; de Almeida, Nathalia Nogueira da Costa; Cordeiro, Marcela Silva; Santos, Simone do Socorro Damasceno.
Afiliación
  • Homobono BP; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • das Mercês MO; UFRA - Universidade Federal Rural da Amazônia - Estr, Belém - PA, Brazil.
  • Nogueira LHDS; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • de Souza EB; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • Cardoso APL; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • Santos ABS; IFPA - Instituto Federal de Educação, Belém - PA, Brazil.
  • Ramos AS; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • Costa MHP; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • Santana PDPB; UFRA - Universidade Federal Rural da Amazônia - Estr, Belém - PA, Brazil.
  • de Almeida NNDC; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
  • Cordeiro MS; IFPA - Instituto Federal de Educação, Belém - PA, Brazil.
  • Santos SDSD; UFPA - Universidade Federal do Pará - R. Augusto Corrêa, Belém - PA, Brazil.
Reprod Domest Anim ; 59(3): e14548, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38459830
ABSTRACT
The in vivo fertilization process occurs in the presence of follicular fluid (FF). The aim of this study was to evaluate the effect of in vitro fertilization medium supplementation with 5% or 10% bovine follicular fluid (BFF) on the production of in vitro bovine embryos. FF was collected from ovarian follicles with a diameter of 8-10 mm, and cumulus-oocyte complexes (COCs) were co-incubated with sperm for 24 h in the commercial medium BotuFIV® (BotuPharma©), being distributed among the experimental groups oocytes fertilized in a control medium; oocytes fertilized in a medium supplemented with 5% BFF; and oocytes fertilized in a medium supplemented with 10% BFF. After fertilization, the zygotes were cultured in vitro for 8 days. Embryo development was assessed through cleavage rates (day 2) and blastocyst formation rates (day 8). The relative expression of the genes OCT4, IFNT2, BAX, HSP70 and SOD2 was measured using the real-time polymerase chain reaction method. There was no difference (p > .05) among the different experimental groups in terms of cleavage rates and blastocyst formation rates. Regarding the gene expression results, only the blastocysts from oocytes fertilized with 10% BFF showed significantly lower expression of IFNT2 (p = .003) and SOD2 (p = .01) genes compared to blastocysts from oocytes fertilized in control medium alone, while there was no difference between blastocyst from oocytes fertilized in control medium and the ones from oocytes fertilized with 5% BFF. In addition to this, the blastocysts from oocytes fertilized with 5% BFF showed significantly reduced levels of expression of the heat shock protein HSP70 (p < .001) and the pro-apoptotic protein BAX (p = .015) compared to blastocysts from oocytes fertilized with control medium. This may indicate that lower supplementation of BFF to the IVF medium creates a more suitable environment for fertilization and is less stressful for the zygote.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Líquido Folicular Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Líquido Folicular Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania