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The effect of mesenchymal stem cells administration on DNA repair gene expressions in critically ill COVID-19 patients: prospective controlled study.
Isiksaçan, Nilgün; Adas, Gökhan; Kasapoglu, Pinar; Çukurova, Zafer; Yilmaz, Rabia; Kurt Yasar, Kadriye; Irmak Koyuncu, Duygu; Tuncel, Fatima Ceren; Sahingöz Erdal, Gülçin; Gedikbasi, Asuman; Pehlivan, Sacide; Karaoz, Erdal.
Afiliación
  • Isiksaçan N; Department of Biochemistry, Bakirköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  • Adas G; Stem Cell And Gene Therapies Application And Research Center, Department Of Surgery, Bakirköy Dr. Sadi Konuk Training And Research Hospital, University Of Health Sciences, Istanbul, Turkey.
  • Kasapoglu P; Department of Biochemistry, Bakirköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  • Çukurova Z; Department of Anesthesia and Intensive Care, Bakirköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  • Yilmaz R; Department of Anesthesia and Intensive Care, Bakirköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  • Kurt Yasar K; Department of Infectious Disease, Istanbul Bakirköy Dr.Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
  • Irmak Koyuncu D; Center of Stem Cells and Tissue Engineering Research & Practice, Istinye University, Istanbul, Turkey.
  • Tuncel FC; Department of Medical Biology Faculty of Medicine, Istanbul University, Istanbul, Turkey.
  • Sahingöz Erdal G; Department of Oncology, Bakirköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  • Gedikbasi A; Department of Pediatric Basic Science, Division of Medical Genetics, Institute of Child Health, Istanbul University, Istanbul, Turkey.
  • Pehlivan S; Department of Medical Biology Faculty of Medicine, Istanbul University, Istanbul, Turkey.
  • Karaoz E; Department of Histology & Embrology, Faculty of Medicine, Istinye University, LIV Hospital, Center of Regenerative Medicine and Stem Cell Manufacturing, Istanbul, Turkey.
Nucleosides Nucleotides Nucleic Acids ; : 1-16, 2024 Mar 09.
Article en En | MEDLINE | ID: mdl-38459810
ABSTRACT
When the studies are evaluated, immunomodulatory effect of MSCs, administration in critically ill patients, obstacle situations in use and side effects, pulmonary fibrosis prevention, which stem cells and their products, regeneration effect, administration route, and dosage are listed under the main heading like. The effect of MSC administration on DNA repair genes in COVID-19 infection is unknown. Our aim is to determine the effect of mesenchymal stem cells (MSCs) therapy applied in critically ill patients with coronavirus infection on DNA repair pathways and genes associated with those pathways. Patients (n = 30) divided into two equal groups. Group-1 Patients in a critically ill condition, Group-2 Patients in critically ill condition and transplanted MSCs. The mechanism was investigated in eleven genes of five different pathways; Base excision repair PARP1, Nucleotide excision repair (NER) RAD23B and ERCC1, Homologous recombinational repair (HR) ATM, RAD51, RAD52 and WRN, Mismatch repair (MMR) MLH1, MSH2, and MSH6, Direct reversal repair pathway MGMT. It was found that MSCs application had a significant effect on 6 genes located in 3 different DNA damage response pathways. These are NER pathway genes; RAD23 and ERCC1, HR pathway genes; ATM and RAD51, MMR pathway genes; MSH2 and MSH6 (p < 0.05). Two main points were shown. First, as a result of cellular damage in critical patients with COVID-19, DNA damage occurs and then DNA repair pathways and genes are activated in reaction to this situation. Second, administration of MSC to patients with COVID-19 infection plays a positive role by increasing the expression of DNA repair genes located in DNA damage pathways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nucleosides Nucleotides Nucleic Acids Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nucleosides Nucleotides Nucleic Acids Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos