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Interkingdom interactions shape the fungal microbiome of mosquitoes.
Hegde, Shivanand; Khanipov, Kamil; Hornett, Emily A; Nilyanimit, Pornjarim; Pimenova, Maria; Saldaña, Miguel A; de Bekker, Charissa; Golovko, George; Hughes, Grant L.
Afiliación
  • Hegde S; Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, UK. s.m.hegde@keele.ac.uk.
  • Khanipov K; School of Life Sciences, Keele University, Newcastle, UK. s.m.hegde@keele.ac.uk.
  • Hornett EA; Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX, USA.
  • Nilyanimit P; Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, UK.
  • Pimenova M; Institute of Infection, Veterinary and Ecological Sciences, Department of Evolution, Ecology and Behaviour, University of Liverpool, Liverpool, UK.
  • Saldaña MA; Center of Excellence in Clinical Virology, Faculty of Medicine , Chulalongkorn University, Bangkok, Thailand.
  • de Bekker C; Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX, USA.
  • Golovko G; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
  • Hughes GL; Microbiology, Department of Biology, Utrecht University, 3584 CH, Utrecht, The Netherlands.
Anim Microbiome ; 6(1): 11, 2024 Mar 07.
Article en En | MEDLINE | ID: mdl-38454530
ABSTRACT

BACKGROUND:

The mosquito microbiome is an important modulator of vector competence and vectoral capacity. Unlike the extensively studied bacterial microbiome, fungal communities in the mosquito microbiome (the mycobiome) remain largely unexplored. To work towards getting an improved understanding of the fungi associated with mosquitoes, we sequenced the mycobiome of three field-collected and laboratory-reared mosquito species (Aedes albopictus, Aedes aegypti, and Culex quinquefasciatus).

RESULTS:

Our analysis showed both environment and host species were contributing to the diversity of the fungal microbiome of mosquitoes. When comparing species, Ae. albopictus possessed a higher number of diverse fungal taxa than Cx. quinquefasciatus, while strikingly less than 1% of reads from Ae. aegypti samples were fungal. Fungal reads from Ae. aegypti were < 1% even after inhibiting host amplification using a PNA blocker, indicating that this species lacked a significant fungal microbiome that was amplified using this sequencing approach. Using a mono-association mosquito infection model, we confirmed that mosquito-derived fungal isolates colonize Aedes mosquitoes and support growth and development at comparable rates to their bacterial counterparts. Strikingly, native bacterial taxa isolated from mosquitoes impeded the colonization of symbiotic fungi in Ae. aegypti suggesting interkingdom interactions shape fungal microbiome communities.

CONCLUSION:

Collectively, this study adds to our understanding of the fungal microbiome of different mosquito species, that these fungal microbes support growth and development, and highlights that microbial interactions underpin fungal colonization of these medically relevent species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Microbiome Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Microbiome Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido