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CRISPR-like sequences association with antibiotic resistance and biofilm formation in Helicobacter pylori clinical isolates.
Yousefi, Leila; Kadkhoda, Hiva; Shirmohammadi, Masoud; Moaddab, Seyyed Yaghoub; Ghotaslou, Reza; Sadeghi, Javid; Somi, Mohammad Hossein; Ahangarzadeh Rezaee, Mohammad; Ganbarov, Khudaverdi; Samadi Kafil, Hossein.
Afiliación
  • Yousefi L; Student Research Committee, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Kadkhoda H; Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Shirmohammadi M; Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Moaddab SY; Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Ghotaslou R; Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Tahereh Pirzadeh; Stem Cell Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Sadeghi J; Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Somi MH; Liver and Gastrointestinal Diseases Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Ahangarzadeh Rezaee M; Stem Cell Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Ganbarov K; Research Laboratory of Microbiology and Virology, Baku State University, Z. Khalilov str., 23 AZ1148, Baku, Azerbaijan.
  • Samadi Kafil H; Drug Applied Research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Heliyon ; 10(5): e26809, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38449645
ABSTRACT
Role of clustered regularly interspaced short palindromic repeats (CRISPR)-like sequences in antibiotic resistance and biofilm formation isn't clear. This study investigated association of CRISPR-like sequences with antibiotic resistance and biofilm formation in H. pylori isolates. Thirty-six of H. pylori isolates were studied for existence of CRISPR-like sequences using PCR method and their correlation with biofilm formation and antibiotic resistance. Microtiter-plate technique was utilized for investigating antibiotic resistance profile of isolates against amoxicillin, tetracycline, metronidazole and clarithromycin. Biofilm formation of isolates was analyzed by microtiter-plate-based-method. Out of 23 CRISPR-like positive isolates, 19 had ability of biofilm formation and 7 of 13 CRISPR-like negative isolates were able to form biofilm (Pvalue = 0.445). In CRISPR-like positive isolates, 11 (48%), 18 (78%), 18 (78%) and 23 (100%) were resistant to amoxicillin, tetracycline, metronidazole and clarithromycin, respectively. Since CRISPR-like sequences have role in antibiotic resistance, may be applied as genetic markers of antibiotic resistance. But there was no substantial correlation between biofilm formation and existence of CRISPR-like sequences. These results indicate possible importance of CRISPR-like sequences on acquisition of resistance to antibiotics in this bacterium.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido