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The Evaluation of Anti-Osteoclastic Activity of the Novel Calcium Hydroxide Biodegradable Nanoparticles as an Intracanal Medicament.
Promta, Patarawadee; Chaiyosang, Patcharaporn; Panya, Aussara; Laorodphun, Pongrapee; Leelapornpisid, Warat; Imerb, Napatsorn.
Afiliación
  • Promta P; Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
  • Chaiyosang P; Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
  • Panya A; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
  • Laorodphun P; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
  • Leelapornpisid W; Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
  • Imerb N; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand. Electronic address: napatsorn.i@cmu.ac.th.
J Endod ; 50(5): 667-673, 2024 May.
Article en En | MEDLINE | ID: mdl-38447912
ABSTRACT

INTRODUCTION:

The aim of this study was to evaluate the anti-osteoclastic activity of calcium hydroxide-loaded poly(lactic-co-glycolic acid) nanoparticles [Ca(OH)2-loaded PLGA NPs] in comparison to calcium hydroxide nanoparticles [Ca(OH)2 NPs].

METHODS:

RAW 264.7 cell lines (third-fifth passage) were cultured and incubated with soluble receptor activator of nuclear factor kappa B ligand in triplicate. Subsequently, Ca(OH)2-loaded PLGA NPs and Ca(OH)2 NPs were added for 7 days to evaluate their effects on receptor activator of nuclear factor kappa B ligand-induced osteoclast differentiation of RAW 264.7 cells by tartrate-resistant acid phosphatase activity. Additionally, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to confirm the cytotoxicity of treatments to cells.

RESULTS:

Tartrate-resistant acid phosphatase staining showed a significant reduction in the osteoclast number when treated with Ca(OH)2-loaded PLGA NPs compared with Ca(OH)2 NPs (P < .01). In comparison to the control, the number of osteoclasts significantly reduced upon treatment with Ca(OH)2-loaded PLGA NPs (P < .05), but there was no significant difference in Ca(OH)2 NPs. Furthermore, osteoclast morphology in both treatment groups exhibited smaller sizes than the control group. Neither Ca(OH)2-loaded PLGA NPs nor Ca(OH)2 NPs demonstrated cytotoxic effects on RAW264.7 cells.

CONCLUSIONS:

Both Ca(OH)2 NPs with and without poly(lactic-co-glycolic acid) have the ability to inhibit osteoclast differentiation. However, Ca(OH)2-loaded PLGA NPs exhibit greater potential than Ca(OH)2 NPs, making them a promising intracanal medicament for cases of root resorption.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoclastos / Hidróxido de Calcio / Nanopartículas / Copolímero de Ácido Poliláctico-Ácido Poliglicólico Límite: Animals Idioma: En Revista: J Endod Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoclastos / Hidróxido de Calcio / Nanopartículas / Copolímero de Ácido Poliláctico-Ácido Poliglicólico Límite: Animals Idioma: En Revista: J Endod Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos